- Open Access
An e-learning application on electrochemotherapy
© Corovic et al; licensee BioMed Central Ltd. 2009
- Received: 29 July 2009
- Accepted: 20 October 2009
- Published: 20 October 2009
Electrochemotherapy is an effective approach in local tumour treatment employing locally applied high-voltage electric pulses in combination with chemotherapeutic drugs. In planning and performing electrochemotherapy a multidisciplinary expertise is required and collaboration, knowledge and experience exchange among the experts from different scientific fields such as medicine, biology and biomedical engineering is needed. The objective of this study was to develop an e-learning application in order to provide the educational content on electrochemotherapy and its underlying principles and to support collaboration, knowledge and experience exchange among the experts involved in the research and clinics.
The educational content on electrochemotherapy and cell and tissue electroporation was based on previously published studies from molecular dynamics, lipid bilayers, single cell level and simplified tissue models to complex biological tissues and research and clinical results of electrochemotherapy treatment. We used computer graphics such as model-based visualization (i.e. 3D numerical modelling using finite element method) and 3D computer animations and graphical illustrations to facilitate the representation of complex biological and physical aspects in electrochemotherapy. The e-learning application is integrated into an interactive e-learning environment developed at our institution, enabling collaboration and knowledge exchange among the users. We evaluated the designed e-learning application at the International Scientific workshop and postgraduate course (Electroporation Based Technologies and Treatments). The evaluation was carried out by testing the pedagogical efficiency of the presented educational content and by performing the usability study of the application.
The e-learning content presents three different levels of knowledge on cell and tissue electroporation. In the first part of the e-learning application we explain basic principles of electroporation process. The second part provides educational content about importance of modelling and visualization of local electric field in electroporation-based treatments. In the third part we developed an interactive module for visualization of local electric field distribution in 3D tissue models of cutaneous tumors for different parameters such as voltage applied, distance between electrodes, electrode dimension and shape, tissue geometry and electric conductivity. The pedagogical efficiency assessment showed that the participants improved their level of knowledge. The results of usability evaluation revealed that participants found the application simple to learn, use and navigate. The participants also found the information provided by the application easy to understand.
The e-learning application we present in this article provides educational material on electrochemotherapy and its underlying principles such as cell and tissue electroporation. The e-learning application is developed to provide an interactive educational content in order to simulate the "hands-on" learning approach about the parameters being important for successful therapy. The e-learning application together with the interactive e-learning environment is available to the users to provide collaborative and flexible learning in order to facilitate knowledge exchange among the experts from different scientific fields that are involved in electrochemotherapy. The modular structure of the application allows for upgrade with new educational content collected from the clinics and research, and can be easily adapted to serve as a collaborative e-learning tool also in other electroporation-based treatments such as gene electrotransfer, gene vaccination, irreversible tissue ablation and transdermal gene and drug delivery. The presented e-learning application provides an easy and rapid approach for information, knowledge and experience exchange among the experts from different scientific fields, which can facilitate development and optimisation of electroporation-based treatments.
- Local Electric Field
- Educational Content
- Gene Electrotransfer
- Pedagogical Efficiency
- Tissue Electroporation
Electrochemotherapy is an effective approach in tumor treatment employing locally applied high-voltage electric pulses in combination with chemotherapeutic drugs which enter tumor cells after their membrane has been electroporated [1, 2]. Electroporation is a phenomenon of cell membrane permeability increase due to local delivery of short and sufficiently intense voltage pulses via appropriate electrodes to the target cells and tissues [3, 4]. In addition to electrochemotherapy, other medical applications of electroporation are emerging at an increasing rate, such as gene electrotransfection [5, 6], cell fusion  and irreversible tissue ablation  and transdermal gene and drug delivery . The effectiveness of cell and tissue electroporation, and thus the effectiveness of electroporation-based therapies, depends on one hand on the parameters of the applied pulses such as amplitude, duration, number and repetition frequency and type of electrodes used and on the other hand on the characteristics of the cell and tissues to be electroporated. Depending on the electric pulse parameters used, electroporation can be reversible or irreversible. Namely, when the electric pulses are applied, local electric field (E) is established within the treated tissue. In order to cause structural changes in cell membrane magnitude of local electric field need to achieve the critical reversible threshold value (Erev). The phenomenon is reversible until the magnitude of local electric field reaches the irreversible threshold value Eirrev, which causes permanent damages of the cell membrane. The reversible electroporation regime has to be assured in all applications in which the viability of cells has to be preserved, such as electrochemotherapy and particularly gene therapy . On the other hand, in some medical and biotechnological applications such as irreversible tumour tissue ablation, liquid food sterilization or water treatment, the irreversible electroporation is used as a nonthermal method for efficient cell killing . The key role in electroporation effectiveness plays the local electric field, which can be directly modified by the amplitude of delivered electric pulses and electrodes used for electric pulse delivery . Thus, for controlled use of the method in each particular electroporation-based application electric pulse parameters and electrodes' shape and placement with respect to the target tissue need to be specifically optimized .
Knowledge exchange and collaboration among the experts involved in electroporation-based therapies
Scientific fields and the corresponding expertise needed in electrochemotherapy
Tumor cells and tissues, cancer
Cells, normal tissue
Physics of biological cells and tissues
- Physical chemistry:
- Biophysical engineering:
Application of physics in medicine and biology
- Computer engineering:
Database systems, interactive web applications
Information and communication technology is necessary for efficient interdisciplinary collaboration and knowledge exchange. Internet technology has already been successfully used to support clinical trials of electrochemotherapy by establishing a central database and the Web application system for electronic collection of data (such as treatment parameters used and treatment efficiency follow up) submitted by users from distant medical centres across Europe [13–15]. Based on a comprehensive analysis of collected data, performed by the developed system the standard operating procedures for clinical electrochemotherapy of cutaneous and subcutaneous tumor in patients have been defined [2, 16–18]. The clinical trials showed and numerous other studies demonstrated, that electrochemotherapy is an efficient antitumor treatment regardless of tumor histology and its location. In order to further improve the treatment planning methods also for other electroporation-based therapies, to develop the needed equipment (i.e. generators, electrodes, software) and to broaden the clinical electrochemotherapy to other types of tumours, numerous international and multidisciplinary scientific projects are being conducted.
A collaborative e-learning in electrochemotherapy
The objective of our study was to develop an e-learning application to support collaboration, knowledge and experience exchange among experts involved in electrochemotherapy and to also apply the acquired knowledge to other electroporation-based technologies such as gene electrotransfection, irreversible tissue ablation and transdermal gene and drug delivery. The target users of our application are biomedical engineers, biologists involved in research and other application development, the clinicians, oncologists and medical personnel involved in choosing and performing the treatment, but also patients and all those who want to learn about electrochemotherapy. The target audience is therefore mixed  (i.e. coming from scientific areas, different fields of expertise, and with different level of experiences) and dispersed  (i.e. geographically located in different research centres spread around Europe/World). In order to consider the users involved in electrochemotherapy our e-learning application was designed to provide educational material for collaborative and flexible learning.
Computer-supported learning of various types i.e. e-learning based educational trainings such as web-based learning, CD-contents or virtual instruments play an important roll in sharing learning content and educational materials, which brings new potential for interdisciplinary and international co-operation among experts from different fields . The e-learning programs that incorporate computer based simulations and visualization tools enable educationally effective and enjoyable learning and teaching methods compared to the conventional learning methods such as learning through listening to spoken words [22, 23]. The use of computer based simulation techniques are particularly important in developing active e-learning environments and "hands-on" e-learning activities, which is proven to be important component in electromagnetic engineering, biomedical engineering and medical education [24–26]. In designing the e-learning content when the target users are coming from different professional backgrounds and with different levels of knowledge it is essential to develop an adaptive interface which can be suitable for different categories of users: novices, intermediates or expert users. In order to more clearly represent the underling mechanisms from the engineering, biological, chemical and medical sciences, scientific and information visualization concepts based on computer graphics software are necessary [27, 28]. Furthermore, collaboration, learning, networking, communication of scientific ideas and knowledge and experience exchange, among the mixed and dispersed audience can be facilitated by computer-supported collaborative visualization [29, 30].
The web based technologies facilitate flexible learning by providing a choice of learning modalities (i.e. in local, near or remote conditions), which is particularly important when the dispersed audience is concerned . Accordingly, we used web-based technologies to collect, organize and transfer the acquired knowledge among the target audience in electrochemotherapy. We used computer graphics such as model-based visualization and simple 2D and 3D computer animations and graphical illustrations to facilitate the representation of complex biological and physical mechanisms involved in electrochemotherapy. The educational content is based on previously published results from molecular dynamics, lipid bilayers, single cell level and simplified tissue models to complex biological tissues [3, 4, 11, 31–43].
The e-learning application is integrated into an interactive e-learning environment E-CHO  developed at our institution. The e-learning application on electrochemotherapy was introduced to the participants at the International Scientific workshop and postgraduate course (Electroporation Based Technologies and Treatments) that took place at the University of Ljubljana in November 2007 . The pedagogical efficiency of the application was analyzed by participant evaluation on the presented educational content at the beginning and at the end of the e-learning training session. We also present the results of a simple usability evaluation of the application we performed by asking the participants to answer to a usability questionnaire and to provide users opinion/comments on the application and suggestions on its possible improvement.
Denmark (University of Copenhagen: 1 biologist (PhD student) and 1 medical physician (PhD researcher) from Herlev Hospital and 1 from Gentofte Hospital);
France (1 physicist (PhD student) from doctoral school École normale supérieure de Cachan; 1 biologist (PostDoc researcher) from Institut Gustave Roussy, Villejuif; 2 biologists (1 PhD student and 1 PostDoc researcher) from IPBS (Institut de Pharmacologie et de Biologie Structurale) - Research Unit of CNRS/UMR 5089 and University Paul Sabatier, Toulouse);
Egypt (University of Cairo: 1 physicist (PhD student) from Biophysics Department, Faculty of Science); and
Slovenia (University of Ljubljana: 8 electrical engineers (PhD students) from Faculty of Electrical Engineering and 1 biologist (PhD student) from Faculty of Pharmacy).
In order to statistically analyze the obtained results we divided the mixed audience/participants into two groups:
first group of 11 engineers (by gathering electrical engineers and physicists) and second group of 7 biologists (by gathering biologists and the medical physician).
The participants were gathered in a computer-based classroom providing each participant with a computer. Each of the participants was provided with a username and password to log on to the E-CHO system. Before the start of the e-learning session a Power Point presentation was presented to the participants by the instructor giving instructions on the course of studying the educational content and on the evaluation testing. In order to create a collaborative e-learning environment the participants were encouraged to collaborate (i.e. discuss between each other and with the instructor) while studying the educational content.
The participants were given the instruction to execute the e-learning session according to the linear sequence of studying steps  by starting at the beginning of the e-learning content and by concluding with the final evaluation tests. The evaluation tests were taken by each of the participants only once.
1) Pedagogical efficiency study
In order to evaluate the pedagogical efficiency of the educational content on electrochemotherapy the participants were asked to answer to the same test before and at the end of the e-learning session. The questions were targeted so as to give 50% to 100% success. The exact questions asked in the pre and post e-learning session test are given in Additional file 1.
2) Usability study
The usability evaluation was conducted at the end of the e-learning session after the pedagogical efficiency evaluation was completed. The participants were asked to complete a usability questionnaire related to the user satisfaction with the developed e-learning application, in order to allow the authors (i.e. developers and instructors) to detect possible errors or to obtain the users feedback on further upgrades/improvements. The questionnaire consisted of thirteen usability related questions (see Additional file 2). The participants were asked to express their opinion on a seven point Likert scale (LS) ranging from 1 (disagree - LS (1)) to 7 (strongly agree (LS - (7)) statement or to remain neutral by checking neither agree nor disagree (NA) statement, which we considered as negative evaluation result (Additional file 2). After completing the usability questionnaire the participants were encouraged to provide their opinion/comments on the application and suggestions for its improvement.
The structure of the e-learning content
By using simple graphical illustration we pointed out that the effectiveness of electrochemotherapy can be improved by: optimizing the applied voltage, changing electrode dimension or changing electrode orientation and their position, which we previously predicted by means of numerical modelling. We further provide a list of important parameters of the local electric field in electroporation-based treatments, such as: electrode geometry (needle or plate electrodes), dimension of the particular electrode (width, length, diameter), distance between electrodes, electrode position with respect to the target tissue, electrode orientation with respect to the target tissue, geometry of the target tissue, geometry of the tissue surrounding the target tissue, the contact surface between the electrode and the tissue, electric properties of the target tissue i.e. tissue conductivity, electric properties of the surrounding tissue, the voltage applied to the electrodes and threshold values of the tissue Erev and Eirrev. Using mathematical modelling and graphical illustrations we showed that the local electric field within the treated tissue is not homogeneous due to the specific structure and electric properties of the tissues (particularly of the target tumour tissue that usually has higher electric conductivity than its surrounding tissues).
In the third part of the e-learning application (Local electric field in 3D tissue models) we developed an interactive module for visualization of local electric field distribution in tissues for different parameters such as voltage applied, distance between electrodes, electrode' dimension and shape, tissue geometry and electric conductivity. The module provides 3D animations we developed by using 3D Studio Max, which were based on previously calculated local electric field distribution in 3D realistic tissue models. For the numerical calculations we used COMSOL Multiphysics software.
The module allows for local electric field visualization in cutaneous (protruding tumours) and subcutaneous tumours (tumours more deeply seeded in the tissue). Users can appreciate the local electric field distribution within the treated tissue when electroporated directly or through the skin by using plate or needle electrodes. The module also provides a guideline on how to overcome a highly resistive skin tissue in order to permeabilize more conductive underlying tissues.
in order to successfully electroporate the target tumour through the skin layer a higher voltage needs to be applied compared to the tumour electroporation, which further depends also on skin thickness. The user is offered a guideline on how to overcome the highly resistive skin tissue in order to permeabilize more conductive underlying tissues using plate electrodes (Fig. 8);
plate electrodes are more suitable for treatment of protruding cutaneous tumours, while for situations when the tumour is seeded more deeply in the tissue needle electrodes are to be used (Fig. 9a and 9b), and;
by increasing the number of needle electrodes stronger local electric field in the tissue can be achieved (Fig. 9c).
The educational web pages are concluded by a test (see Additional file 1) that gives the user an opportunity to test the acquired knowledge, while allowing the teacher and the web-developer to follow the efficacy of the constructed pages and their educational success.
Results of the pedagogical efficiency evaluation
for biologists the average percentage rates of correct answers changed from 73% before e-learning session to 87% after e-learning session, with a large dispersion depending on the question (for example - question 4: from 0% before to 43% after e-learning session; question 9 from 86% before to 100% after e-learning session), Fig. 10b.
for engineers the average percentage rates of correct answers changed from 89% before e-learning session to 95% after e-learning session, Fig. 10c.
Results of the usability evaluation
The participants evaluated the statement that the information provided by the system is easy to understand (question 9) with the highest percentage of agree statements in the Likert scale (58.3% of LS (7) and 25% of LS (6)). Participant were most neutral (41.7% of NA) regarding question 12 (The system covers all the areas I expected to cover). However, the same question was evaluated with 50% of LS (6) and 8.3% of LS (7) statements. The participants were neutral with 33% for questions 6 (I believe I became more confident with the system) (with 50% of LS (6)) and for question 11 (The interface of system is pleasant) (with 41.67% of LS (6)). Overall, the participants were satisfied with the developed e-learning application (question 13) with 41.6% of the highest percentage of agree statements in the Likert scale (LS (7)) and with only 8.3% of neutral statements (NA). The results of the usability evaluation (Fig. 11) also revealed that the participants were satisfied with how easy it was to use the system (question 1: 33.3% of LS (7) and 50% of LS (6)). The participants were comfortable using the system (question 4: 33.33% of LS (7) and 50% of LS (6)) and found the system simple to use (question 2: 41. 67% of both LS (7) and LS (6)), to learn to use (question 5: 41.67% of LS (7) and 50% of LS(6)) and to be effectively navigated (question 3: 25% of LS (7) and 66.67% of LS (6)). The users also found the information provided with system (such as online help, on-screen messages, and other documentation) clear (question 7: 33.3% of LS (7) and 50% of LS (6)), easy to find (question 8: 25% of LS (7) and 50% of LS (6)) and effective and complete (question 10: 16.67% of LS (7) and 66.67% of LS (6)) (Fig. 11).
After completing the usability questionnaire the participants provided their opinion/comments on the application and suggestions on its improvement. Most of participants provided the comment that they liked the idea to present the knowledge on electrochemotherapy in the form of e-learning application. The participants particularly found interesting the interactive visualization of local electric field in tissues for different parameters such as voltage applied, distance between electrodes, electrode' dimension, which for the time being can not be visualized while performing the electrochemotherapy treatment. The engineers, who are not familiar with chemical and biological processes during electroporation of cells and tissues, suggested that more of biological and chemical background should be also added to the existing educational material. On the other hand the biologists suggested that it would be interesting to have a possibility to visualize the distribution of local electric field and changes in electric properties for different cell types such as muscle fibers, hepatocytes, blood vessels, while being electroporated and which are potential target cells for gene transfer.
We developed, implemented and evaluated an e-learning application on electroporation-based therapies such as electrochemotherapy. This is the first e-learning application developed to support collaboration, knowledge and experience exchange among the experts from different scientific fields involved in electrochemotherapy and other electroporation-based therapies and in order to organize and to transfer the acquired knowledge and experience to the users (such as clinicians, medical personnel, students, patients and all those who want to learn about electroporation-based therapies).
The educational content on electrochemotherapy and cell and tissue electroporation is based on previously published studies from molecular dynamics, lipid bilayers, single cell level and simplified tissue models to complex biological tissues and research and clinical results of electrochemotherapy treatment [3, 4, 11, 31–43].
The e-learning content presents three different levels of knowledge on cell and tissue electroporation. In the first part of the e-learning application we explain basic mechanisms underlying electroporation process. Based on simple graphical illustrations we demonstrated the influence of each of the pulse parameters, such as pulse amplitude, pulse number and duration, on electroporation of cells with different sizes, shapes and orientations with respect to the applied electric field. By using 3D animation we visualized the aqueous pore formation in cell membrane, which is most widely accepted model, among different theoretical models that describe cell membrane electroporation.
Electrochemotherapy treatment outcome is directly related to the local electric field distribution within the target tumour tissue and its surrounding tissues [11, 12, 40, 47–49]. The second part of the e-learning content was thus developed in order to provide the educational material about the parameters of local electric field being crucial to make the tumor treatment as efficient as possible. For this purpose we used combination of numerical calculations by means of mathematical modelling and simple graphical illustrations. We demonstrated how the pulse amplitude, electrode shape and electrode positioning influence on the local electric field distribution within the treated cells and tissues. We also demonstrated how the electric properties of a treated sample (i.e. its geometry and electric conductivity) can modify the local electric field distribution. Namely, when the voltage is applied, the electric field distributes within the complex tissue with different electric properties as in voltage divider. The latter means that the electric field is the highest in the layer with the highest electric resistivity (lowest conductivity) , which is particularly important when electroporating the skin and/or its underlying tissues.
In the third part of the e-learning application, we developed an interactive module for visualization of local electric field distribution in tissues for different parameters such as voltage applied, distance between electrodes, electrode' dimension, tissue geometry and electric conductivity. The interactive module is aimed at hands-on learning on how the above-mentioned parameters can modify the local electric field distribution within the treated tissue. The module allows for local electric field visualization in cutaneous and subcutaneous tumours. Users can appreciate the local electric field distribution within the treated tissue when electroporated directly or through the skin by using plate or needle electrodes. The module also provides guidelines on how to overcome a highly resistive skin tissue in order to permeabilize more conductive underlying tissues. Since, for the time being the local electric field in the treated tissue can not be visualized while performing the electrochemotherapy treatment, the interactive visualization approach we provide in our e-learning application can serve as an important tool in selection of the appropriate electric pulses amplitude, electrode shape and their placement with respect to the tissue geometry and its electric conductivity, which is needed for best electrochemothertapy treatment outcome.
Good collaboration among the participants and with the instructor was established during the e-learning session. Namely, the participants assisted each other while studying the educational content and several discussions were initiated between physicists and biologists and between the participants and the instructor. The e-learning application was concluded by a test on the presented educational material and by a questionnaire on usability of the developed application.
We evaluated the designed e-learning application at the International Scientific workshop and postgraduate course (Electroporation Based Technologies and Treatments) . The evaluation was carried out by testing the pedagogical efficiency of the presented educational content and by performing the usability study of the application. The pedagogical efficiency assessment showed that the participants improved their level of knowledge (Fig. 10).
The percentage rate of correct answers for all participants (mixed population) obtained after the e-learning session was above 50% for all test questions (Fig. 10a). The results in Fig. 10b show that before the e-learning session the knowledge of biologists was more heterogeneous compared to the knowledge possessed by engineers as shown in Fig. 10c. This is in part because the level of knowledge possessed by biologists (compared to the engineers) was lower before the e-learning session, since the test and the e-learning content was about electrical parameters. However, the increase in percentage rate of correct answers, after the e-learning session compared to the results obtained before the e-learning session, to each of the questions was obtained for both biologists and engineers (Figs. 10b and 10c). Only for question 4 the percentage of correct answers given by biologist after the e-learning session was slightly below 50% (i.e. 43% of success rate after e-learning session compared to 0% before e-learning session). In order to further improve the success rate of question 4 we concluded that: 1. question 4 should be more clearly formulated by developers and 2. more of e-learning content on the voltage applied between electrodes (U) and on electroporation threshold of local electric field (E) should be provided in the e-learning application. The same question answered by engineers was 70% of success rate before and after e-learning session.
The results of usability evaluation revealed that participants found the application simple to learn to use and navigate (Fig. 11). Overall, the participants were satisfied with the e-learning application. The participants found the information provided by system easy to understand (question 9 with the highest percentage of agree statements in the Likert scale (58.3% of LS (7)) and 25% of LS (6)). The participants were most neutral regarding the statement that the e-learning application covered all the areas they expected to cover (question 12 evaluated with 41.7% of NA). However, the same question was evaluated with 50% of LS (6) and 8.3% of LS (7) statements. The modular structure of the application allows for upgrade with new educational content collected from the clinics and research, and for the integration of new application modules including computer-supported collaborative visualization being an important component in remote collaboration among the experts . The e-learning application can be used as an education form at both levels: either as a completely independent e-learning form or as an integral part of a blended learning form. The e-learning session can be executed by the users in a linear sequence of studying steps according to the program flow model (i.e. by starting at the beginning of the e-learning content and by concluding with the final evaluation tests) or in a studying sequence which is not previously defined, which can serve as an additional e-learning module of blended learning .
The e-learning application together with E-CHO system is available to the users to provide collaborative and flexible learning in order to facilitate knowledge exchange among the experts from different scientific fields that are involved in electrochemotherapy. The e-learning application is developed to provide an interactive educational content in order to simulate the "hands-on" learning approach about the parameters being important for successful therapy. The e-learning application on electrochemotherapy can be easily adapted to serve as a collaborative e-learning tool also in other electroporation-based treatments such as gene electrotransfer, irreversible tissue ablation or transdermal gene and drug delivery [6, 8, 9, 50]. The presented e-learning application provides an easy and rapid approach for information, knowledge and experience exchange among the experts from different scientific fields, which can facilitate development and optimisation of electroporation-based treatments.
This study was supported by the Slovenian Research Agency and by the European Commission within the 5th framework program under the grants Cliniporator QLK3-1999-00484 and ESOPE QLK3-2002-02003 and within the 6th framework program under the grant ANGIOSKIN LSHB-CT-2005-512127.
- Mir LM: Therapeutic perspectives of in vivo cell electropermeabilization. Bioelectrochemistry 2001, 53: 1–10. 10.1016/S0302-4598(00)00112-4View ArticleGoogle Scholar
- Marty M, Sersa G, Garbay JR, Gehl J, Collins CG, Snoj M, Billard V, Geertsen PF, Larkin JO, Miklavcic D, Pavlovic I, Paulin-Kosir SM, Cemazar M, Morsli N, Soden DM, Rudolf Z, Robert C, O'Sullivan GC, Mir LM: Electrochemotherapy-An easy, highly effective and safe treatement of cutaneous and subcutaneous metastases: Results of ESOPE (European Standard Operating Procedures of Electrochemotherapy) study. Eur J Cancer Suppl 2006, 4: 3–13. 10.1016/j.ejcsup.2006.08.002View ArticleGoogle Scholar
- Mir LM, Bureau M, Gehl J, Rangara R, Rouy D, Caillaud J, Delaere P, Branellec D, Schwartz B, Scherman D: High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc Natl Acad Sci USA 1999, 96: 4262–4267. 10.1073/pnas.96.8.4262View ArticleGoogle Scholar
- Miklavcic D, Semrov D, Mekid H, Mir LM: A validated model of in vivo electric field distribution in tissues for electrochemotherapy and for DNA electrotransfer for gene therapy. Biochim Biophys Acta 2000, 1523: 73–83.View ArticleGoogle Scholar
- Prud'homme G, Glinka Y, Khan A, Draghia-Akli R: Electroporation-enhanced nonviral gene transfer for the prevention or treatment of immunological, endocrine and neoplastic diseases. Curr Gene Ther 2006, 6: 243–273. 10.2174/156652306776359504View ArticleGoogle Scholar
- Andre F, Gehl J, Serša G, Préat V, Hojman P, Eriksen J, Golzio M, Cemazar M, Pavselj N, Rols MP, Miklavcic D, Neumann E, Teissie J, Mir LM: Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin. Human Gene Ther 2008, 19: 1261–1271. 10.1089/hum.2008.060View ArticleGoogle Scholar
- Trontelj K, Reberšek M, Kanduser M, Curin-Serbec V, Sprohar M, Miklavcic D: Optimization of bulk cell electrofusion in vitro for production of human-mouse heterohybridoma cells. Bioelectrochemistry 2008, 74: 124–129. 10.1016/j.bioelechem.2008.06.003View ArticleGoogle Scholar
- Rubinsky J, Onik G, Mikus P, Rubinsky B: Optimal parameters for the destruction of prostate cancer using irreversible electroporation. Journal of Urology 2008, 180: 2668–2674. 10.1016/j.juro.2008.08.003View ArticleGoogle Scholar
- Pavselj N, Miklavcic D: A numerical model of permeabilized skin with local transport regions. IEEE Trans Biomed Eng 2008, 55: 1927–1930. 10.1109/TBME.2008.919730View ArticleGoogle Scholar
- Kanduser M, Miklavcic D: Electroporation in biological cell and tissue: an overview. In Electrotechnologies for Extraction from Food Plants and Biomaterials. Edited by: Vorobiev E, Lebovka N. New York, Springer Science; 2008:1–37.Google Scholar
- Miklavcic D, Beravs K, Semrov D, Cemazar M, Demsar F, Sersa G: The importance of electric field distribution for effective in vivo electroporation of tissues. Biophys J 1998, 74: 2152–2158. 10.1016/S0006-3495(98)77924-XView ArticleGoogle Scholar
- Zupanic A, Corovic S, Miklavcic D: Optimization of electrode position and electric pulse amplitude in electrochemotherapy. Radiol Oncol 2008, 42: 93–101. 10.2478/v10019-008-0005-5View ArticleGoogle Scholar
- Pavlovic I, Kramar P, Corovic S, Cukjati D, Miklavcic D: A web-application that extends functionality of medical device for tumor treatment by means of electrochemotherapy. Radiol Oncol 2004, 38: 49–54.Google Scholar
- Pavlovic I, Miklavcic D: Web-based electronic data collection system to support electrochemotherapy clinical trial. IEEE Trans Inf Technol Biomed 2007, 11: 222–230. 10.1109/TITB.2006.879581View ArticleGoogle Scholar
- Pavlovic I, Kern T, Miklavcic D: Comparison of paper-based and electronic data collec-tion process in clinical trials: Costs simulation study. Contemp Clin Trials 2009, 30: 300–316. 10.1016/j.cct.2009.03.008View ArticleGoogle Scholar
- Mir LM, Gehl J, Sersa G, Collins CG, Garbay JR, Billard V, Geertsen P, Rudolf Z, O'Sullivan GC, Marty M: Standard operating procedures of the electrochemotherapy: instructions for the use of bleomycin or cisplatin administered either systemically or locally and electric pulses delivered by the CliniporatorTM by means of invasive or non-invasive electrodes. Eur J Cancer Suppl 2006, 4: 14–25. 10.1016/j.ejcsup.2006.08.003View ArticleGoogle Scholar
- Sersa G, Miklavcic D: Electrochemotherapy of tumours (Video Article). J Visual Exp 2008, 22: 1038.Google Scholar
- Colombo GL, Di Matteo S, Mir LM: Cost-effectiveness analysis of electrochemotherapy with the Cliniporator™ vs other methods for the control and treatment of cutaneous and subcutaneous tumors. Therapeutics and Clinical Risk Management 2008, 2: 541–548.Google Scholar
- Godfrey M: Teaching software engineering to a mixed audience. Information and Software Technology 1998, 40: 229–232. 10.1016/S0950-5849(98)00043-3View ArticleGoogle Scholar
- Gillet D, Nguyen Ngoc AV, Rekik Y: Collaborative web-based experimentation in flexible engineering education. IEEE Trans Educ 2005, 48: 696–703. 10.1109/TE.2005.852592View ArticleGoogle Scholar
- Holbert KE, Karady GG: Strategies, challenges and prospects for active learning in computer-based classroom. IEEE Trans Educ 2009, 52: 31–38. 10.1109/TE.2008.917188View ArticleGoogle Scholar
- Dale E: Audio-Visual methods in teaching. 3rd edition. New York, Holt, Rinehart, and Winston; 1969.Google Scholar
- Day AJ, Foley DJ: Evaluating a web lecture intervention in a human-computer interaction course. IEEE Trans Educ 2006, 49: 420–431. 10.1109/TE.2006.879792View ArticleGoogle Scholar
- Buret F, Muller D, Nicolas L: Computer-aided education for magnetostatics. IEEE Trans Educ 1999, 42: 45–49. 10.1109/13.746334View ArticleGoogle Scholar
- Guerrero JF, Bataller M, Soria E, Magdalena R: BioLab: An educational tool for signal processing training in biomedical engineering. IEEE Trans Educ 2007, 50: 34–40. 10.1109/TE.2006.886463View ArticleGoogle Scholar
- Biomedical sciences teaching modules[http://www.virtualcampus.ch/display.php?lang=1&pname=discipline_4]
- Telea AC: Data visualization - principles and practice. Wellesley, Massachusetts, AK Peters Ltd; 2008.Google Scholar
- Assaad RS, Silva-Martinez J: A graphical approach to teaching amplifier design at the undergraduate level. IEEE Trans Educ 2009, 52: 39–45. 10.1109/TE.2008.917190View ArticleGoogle Scholar
- Teyseyre AR, Campo MR: An overview of 3D software visualization. IEEE Trans Vis Comput Graphics 2009, 15: 87–105. 10.1109/TVCG.2008.86View ArticleGoogle Scholar
- Hoic-Bozic N, Mornar V, Boticki I: A blended learning approach to course design and implementation. IEEE Trans Educ 2009, 52: 19–30. 10.1109/TE.2007.914945View ArticleGoogle Scholar
- Neumann E, Rosenheck K: Permeability changes induced by electric impulses in vesicular membranes. J Membr Biol 1972, 10: 279–290. 10.1007/BF01867861View ArticleGoogle Scholar
- Tsong TY: Electroporation of cell membranes. Biophys J 1991, 60: 297–306. 10.1016/S0006-3495(91)82054-9View ArticleGoogle Scholar
- Weaver JC, Chizmadzhev YA: Theory of electroporation: a review. Bioelectrochem Bioenerg 1996, 41: 135–160. 10.1016/S0302-4598(96)05062-3View ArticleGoogle Scholar
- Teissie J, Eynard N, Gabriel B, Rols MP: Electropermeabilization of cell membranes. Adv Drug Deliver Rev 1999, 35: 3–19. 10.1016/S0169-409X(98)00060-XView ArticleGoogle Scholar
- Kotnik T, Miklavcic D: Analytical description of transmembrane voltage induced by electric fields on spheroidal cells. Biophys J 2000, 79: 670–679. 10.1016/S0006-3495(00)76325-9View ArticleGoogle Scholar
- Valic B, Pavlin M, Miklavcic D: The effect of resting transmembrane voltage on cell electropermeabilization: a numerical analysis. Bioelectrochemistry 2004, 63: 311–315. 10.1016/j.bioelechem.2003.12.006View ArticleGoogle Scholar
- Pavlin M, Kanduser M, Rebersek M, Pucihar G, Hart FX, Magjarevic R, Miklavcic D: Effect of cell electroporation on the conductivity of a cell suspension. Biophys J 2005, 88: 4378–4390. 10.1529/biophysj.104.048975View ArticleGoogle Scholar
- Tarek M: Membrane electroporation: a molecular dynamics simulation. Biophys J 2005, 88: 4045–4053. 10.1529/biophysj.104.050617View ArticleGoogle Scholar
- Sel D, Cukjati D, Batiuskaite D, Slivnik T, Mir LM, Miklavcic D: Sequential finite element model of tissue electropermeabilization. IEEE Trans Biomed Eng 2005, 52: 816–827. 10.1109/TBME.2005.845212View ArticleGoogle Scholar
- Pavselj N, Bregar Z, Cukjati D, Batiuskaite D, Mir LM, Miklavcic D: The course of tissue permeabilization studied on a mathematical model of a subcutaneous tumor in small animals. IEEE Trans Biomed Eng 2005, 52: 1373–1381. 10.1109/TBME.2005.851524View ArticleGoogle Scholar
- Kotnik T, Miklavcic D: Theoretical evaluation of voltage inducement on internal membranes of biological cells exposed to electric fields. Biophys J 2006, 90: 480–491. 10.1529/biophysj.105.070771View ArticleGoogle Scholar
- Teissié J, Escoffre JM, Rols MP, Golzio M: Time dependence of electric field effects on cell membranes. A review for a critical selection of pulse duration for therapeutical applications. Radiol Oncol 2008, 42: 196–206. 10.2478/v10019-008-0016-2View ArticleGoogle Scholar
- Pavselj N, Miklavcic D: Numerical modeling in electroporation-based biomedical applications. Radiol Oncol 2008, 42: 159–168. 10.2478/v10019-008-0008-2View ArticleGoogle Scholar
- Interactive e-learning system (E-CHO), Laboratory of telecommunications, Faculty of Electrical Engineering, University of Ljubljana[http://www.ltfe.org]
- International Scientific workshop and postgraduate course (Electroporation Based Technologies and Treatments), Faculty of Electrical Engineering, University of Ljubljana[http://www.cliniporator.com/ect]
- Humar I, Sinigoj A, Bester J, Hegler OM: Integrated component web-based interactive learning systems for engineering. IEEE Trans Educ 2005, 8: 664–675. 10.1109/TE.2005.858396View ArticleGoogle Scholar
- Corovic S, Zupanic A, Miklavcic D: Numerical modeling and optimization of electric field distribution in subcutaneous tumor treated with electrochemotherapy using needle electrodes. IEEE Trans Plasma Sci 2008, 36: 1665–1672. 10.1109/TPS.2008.2000996View ArticleGoogle Scholar
- Corovic S, Al Sakere B, Haddad V, Miklavcic D, Mir LM: Importance of contact surface between electrodes and treated tissue in electrochemotherapy. Technol Cancer Res Treat 2008, 7: 393–399.View ArticleGoogle Scholar
- Corovic S, Mojca P, Miklavcic D: Analytical and numerical quantification and comparison of the local electric field in the tissue for different electrode configurations. Biomed Eng Online 2007, 37: 1–14.Google Scholar
- Al-Sakere B, Bernat C, Andre F, Connault E, Opolon P, Davalos R, Mir LM: A study of the immunological response to tumor ablation with irreversible electroporation. Technol Cancer Res Treat 2007, 6: 301–305.View ArticleGoogle Scholar
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