Table 1 Final panel of antibodies
From: Computational flow cytometric analysis to detect epidermal subpopulations in human skin
| Â | Characteristic being measured | Alternative name | Antibody name | Vendor catalog # | Purpose |
|---|---|---|---|---|---|
(clone name) | (dilution used) | ||||
BV421 | Cell surface protein | c-kit | CD117 | BD#562,434 | Keratinocytes in rete ridges (Jiang et al. [7]) |
(YB5B8) | 1:25 | ||||
BV510 | Cell surface protein | MU138 | CD146 | BD#563,255 | Epidermal appendage (Schon et al. [31]) |
(P1H12) | 1:100 | ||||
BV605 | Cell surface protein | Heat-stable antigen(HSA) | CD24 | Biolegend#311,123 | Differentiated keratinocytes (Thierry Magnaldo [34]) |
(ML5) | 1:25 | ||||
APC | Cell surface protein | Integrin β1 | CD29 | BD#559,883 | Basal cells (Kaur and Li 2000) |
(MAR4) | 01:12.5 | ||||
APC-Cy7 | Cell surface protein | Leukocyte common antigen (LCA) | CD45 | BD#557,833 | Leukocytes |
(2D1) | 1:50 | ||||
PE | Cell surface protein | Integrinα6 | CD49f | BD#555,736 | Epidermal basal cells (Kaur and Li 2000) |
(GoH3) | 1:25 | ||||
PE-Cy7 | Cell surface protein | My10 | CD34 (581) | BD#580,710 | Outer root sheath of anagen hair follicles cells (Poblet et al. [35]) |
1:25 | |||||
FITC | Cell surface protein | Â | TLR7 | Novus#NBP2-24,906 | Basal stem cell (Yin et al. [38]) |
1:100 | |||||
PI | Double-stranded DNA | Â | Â | Keygen#KGA106 | Â |
1:500 |