Figure 4
The effect of conjugation under laser scanning confocal microscope and the amount of conjugation. A: FITC-Ahx-GRGDSPC peptide conjugated on PEG-DAV under laser scanning confocal microscope (×200) Scale bars, 50 μm,meanwhile,the negative control appears few fluorescence. B: The quantity (mg) of GRGDSPC peptide in various concentrations immobilized on PEG-cross-linked decellularized valves at a different time points(a) and its proportion in the original GRGDSPC peptide (%)(b) C: VEGF165 conjugated on PEG-DAV under laser scanning confocal microscope (×200)Scale bars, 50 μm, meanwhile, the negative control appears few fluorescence D: The quantity (pg) of rh VEGF165 in various concentrations immobilized on PEG-cross-linked decellularized valve at different points(α) and its proportion in the original rh VEGF165 (%)(β) E: The express of GRGDSPC peptide (green) and VEGF165 (red) conjugated on DAV (4-6) and PEG-DAV (1-3) (×200) 1 and 4 are the express of GRGDSPC peptide, 2 and 5 are the express of VEGF165, 3 and 6 are the simultaneous express of GRGDSPC peptide and VEGF165. Scale bars, 50 μm.