Patterned thin metal film for the lateral resolution measurement of photoacoustic tomography
© Kim et al.; licensee BioMed Central Ltd. 2012
Received: 30 April 2012
Accepted: 13 July 2012
Published: 13 July 2012
Image quality assessment method of photoacoustic tomography has not been completely standardized yet. Due to the combined nature of photonic signal generation and ultrasonic signal transmission in biological tissue, neither optical nor ultrasonic traditional methods can be used without modification. An optical resolution measurement technique was investigated for its feasibility for resolution measurement of photoacoustic tomography.
A patterned thin metal film deposited on silica glass provides high contrast in optical imaging due to high reflectivity from the metal film and high transmission from the glass. It provides high contrast when it is used for photoacoustic tomography because thin metal film can absorb pulsed laser energy. An US Air Force 1951 resolution target was used to generate patterned photoacoustic signal to measure the lateral resolution. Transducer with 2.25 MHz bandwidth and a sample submerged in water and gelatinous block were tested for lateral resolution measurement.
Photoacoustic signal generated from a thin metal film deposited on a glass can propagate along the surface or through the surrounding medium. First, a series of experiments with tilted sample confirmed that the measured photoacoustic signal is what is propagating through the medium. Lateral resolution of the photoacoustic tomography system was successfully measured for water and gelatinous block as media: 0.33 mm and 0.35 mm in water and gelatinous material, respectively, when 2.25 MHz transducer was used. Chicken embryo was tested for biomedical applications.
A patterned thin metal film sample was tested for its feasibility of measuring lateral resolution of a photoacoustic tomography system. Lateral resolutions in water and gelatinous material were successfully measured using the proposed method. Measured resolutions agreed well with theoretical values.
Photoacoustic (opto-acoustic) imaging is an imaging modality which combines the physics of photonics (optics) and ultrasound (acoustics), providing high contrast of optical modality and long signal delivery path of ultrasound . Sub-microsecond optical pulses generate surface or subsurface acoustic ultrasound waves when the pulses are irradiated on photon absorbing structure in biological samples. Generated acoustic waves are collected by an ultrasonic transducer, and then the image is reconstructed by computational procedure. Photoacoustic tomography (PAT) conventionally refers to a photoacoustic imaging technique by which expanded laser irradiation excites a large area of the sample while a moving transducer or multiple array of transducers collect acoustic waves . In contrast to the photoacoustic microscopy (PAM) by which focused laser irradiation excites very small volume of acoustic wave and then is detected by a focused transducer , image reconstruction of PAT resembles that of ultrasonography. For PAM, traditional lateral resolution measurement method using a patterned resolution target such as the US Air Force (USAF) 1951 bar chart has been used .
One of the technical difficulties in PAT system development is the lack of a proper image target with which image quality parameters such as resolution can be adjusted and assessed. Tissue-mimicking phantom development for photoacoustic imaging system has recently been investigated [5, 6], however, resolution targets are not widely adapted yet. There was an attempt to utilize photon-absorbing parallel lines printed on transparency for measurement of lateral resolution of a PAT system . A resolution target for a PAT must contain photon-absorbing fine patterns which are embedded in the optically non-absorbing material. Various PAT image reconstruction algorithms have been developed, which in general rely on analytical solutions to the photoacoustic wave equation assuming the samples are acoustically homogeneous for simplicity . However, difference in acoustic impedance of hetero-materials affects the quality of image reconstruction .
In this study, lateral resolution measurement of a PAT system using a patterned thin metal film will be discussed. Lateral direction in this study refers to the ultrasound propagation direction which is perpendicular to the light propagation. It must be noted that the lateral direction in PAT imaging is the axial direction of reflective ultrasonography.
Identifying ultrasound component
Laser-generated ultrasound in solid has been widely studied since the 1960’s. Different regimens for the production of ultrasound in metals using lasers with wavelengths in visible to near infrared range are summarized extensively in Davies’ review article . Although the propagation of laser-generated ultrasound within the object where it was generated is well understood, its propagation in the surrounding media needs more study. The propagation of laser-generated ultrasound from thin metal film deposited on a base material immersed in water is especially of great interest in this study. Propagation of sound waves in the direction perpendicular to the surface of chromium film from the normal laser irradiation was extensively studied by Ko, et al.[11, 12]. Propagation of sound waves generated in a 0.4-mm-thick stainless steel plate in water for transversal direction was observed by Schlieren imaging . It was demonstrated that various types of sound wave can be formed by different generation mechanisms. The strongest ultrasound signal is generated from the surface vibration in the perpendicular direction to the surface, while weaker ultrasound signal still propagates along the surface mediated by the shear mode of ultrasound in metal .
In this study, we investigate a patterned thin metal film (PTMF) deposited on a transparent plate for its feasibility as a resolution test target for PAT. A commercially available chromium film deposited on a 2-mm fused silica glass with the USAF 1951 resolution bar-chart pattern was used. Positive patterns (Edmund Optics, NT57-896) and negative patterns (Edmund Optics, NT57-895) were used to compare the effect of phase of the generated ultrasound in propagating through a medium. These particular PTMFs are not optimal test targets for PAT because some of their patterns are only suitable for optical characterization. However, they were chosen in this study for their convenience of acquisition. Optimal patterns for PAT will be discussed in Section 4.
where vultrasound represents the speed of sound in the medium. For the PAT system, it is a commonly accepted fact that the lateral resolution of the system is half of Eq. (1) , . The speeds of sound are the material property. Speed of sound in fused silica and in water are ~3,800 m/sec and ~1,500 m/sec, respectively. Since the speed of sound in fused silica is more than twice that in water, the resolution of a PAT system measuring the sound propagating in fused silica will be more than twice lower than that in water. Identifying the correct sound component is of great importance.
Results and discussion
Measurement of lateral resolution in water
The speed of sound in water is ~1,500 m/sec, which gives the theoretical axial resolution of a transducer (2.25 MHz bandwidth) to be 0.33 mm. Signals from Elements 2 – 6 of Group 0 were shown in Figure 4(a). All the elements of Group 0 were distinguished. Element 2 of Group 0 has 1.12 line-pairs-per-mm (lpm), thus the distance between the peaks should be 0.89 mm. After converting the time scale of the data to the distance scale using the speed of sound, the measured distance between the two peaks from Element 2 of Group 0 was 0.90 mm, which is close to the actual value. For a PAT system with lateral resolution of 0.33 mm, three peaks from the element with 2.83 lpm (Element 4 of Group 1) should be distinguishable, while the peaks from the element with 3.17 lpm (Element 5 of Group 1) will start to merge with each other. Figure 4(b) shows the 1D signal from the Group 1 patterns of the same positive PTMF sample. As can be seen from the figure, three diminished peaks are observed in element 4, while only two peaks are observed in element 5. Thus, the PAT system resolution for water is between 0.32 mm and 0.35 mm. When the limited range of patterns is considered, the measured resolution measured from the PTMF is close to the theoretical resolution limit (0.33 mm).
Identifying the sound component
In Section 3.1, the lateral resolution of the PAT system was measured using PTMF samples based on the assumption that the laser-generated ultrasound only propagates in the surrounding water. According to Don-Liyanage’s study , shear wave generated by the surface acoustic wave in solid propagates in all directions. However, compared to the bulk wave which propagates with the same speed in all directions, shear wave has higher speed in the direction parallel to the surface. Thus, if the detected signal is from shear wave, the measured resolution will depend on the angle between the transducer and the surface. If no dependency is observed, then the detected signal is bulk wave.
To identify which component of the sound was detected in this experiment, PTMF sample was tilted by the amount of angle θ (see Figure 2) such that the patterned metal film faced toward the transducer. The transducer axis was still facing toward the center of the PTMF sample. The lateral distance between the bars of the pattern will decrease by the amount of cos(θ). For example, if the tilting angles were θ = 30 degrees and θ = 45 degrees, the minimal spacing of bar patterns that can be distinguished by the PAT system will be 0.38 mm (= 0.33/cos30) and 0.47 mm (= 0.33/cos45), respectively. The minimum spacing resolvable by Elements 3 and 4 of Group 1 ranges from 0.35 mm to 0.40 mm, thus Element 3 or 4 of Group 1 will be able to be distinguished for a 30-degree tilted PTMF sample. The minimum spacing resolvable by Elements 1 and 2 of Group 1 ranges from 0.47 mm to 0.50 mm, thus Element 1 or 2 of Group 1 will be able to be distinguished for a 45-degree tilted PTMF sample.
From the two validation experiments with tilted samples, it is concluded that the ultrasound signals measured in this study demonstrated the same speed regardless of the location of transducer relative to the sample surface where they were measured. It is proven that the observed ultrasound was bulk wave. Intensities of ultrasound signals did not dramatically vary for different transducer locations (sample tilt angles), which is another proof that the observed ultrasound was not shear wave. Spherical bulk wave is the mode of propagating shock wave in this study. The resolution measured by the proposed method is the resolution in the media in which the sample is immersed, rather than the resolution in the sample block.
A practical design of the resolution target for PAT system is discussed. Although it was proven in this study that the patterned thin metal film deposited on a glass plate can be effectively utilized for measuring the lateral resolution of PAT system in a medium, commercially available USAF 1951 target needs modification for practical reasons. First, small patterns designed for optical resolution measurements induce unnecessary interferences as was shown in Figure 3(b). Second, although the speed of sound in biological tissue (for example in liver 1570 m/sec)  is close to that in water (1480 m/sec at room temperature) , there still is discrepancy between two values. A practically useful resolution target for PAT could be made by a PTMF plate having only a limited number of patterns embedded in a transparent material (or tissue-mimicking scattering material) block which has a speed of sound similar to that of biological tissue. The speed of sound in gel material is 1580 m/sec . Resolution of the PAT system for gelatinous material is close to that for some biological tissues.
Patterned thin metal film deposited on fused silica plate was investigated for the measurement of lateral resolution of a PAT system. Propagation of bulk ultrasound wave was confirmed by this proof-of-concept experiment. Lateral resolutions measured by this method for different materials agreed well with theoretical limit. The lateral resolution measured by this method is the resolution of the PAT system in the media rather than the resolution in the sample material. A practically useful resolution target block was suggested and tested. To the best of the authors' knowledge, this finding is the first systematic experimental confirmation using a resolution target showing that the lateral resolution of a PAT system (with a fixed bandwidth of the transducer) is the half of the theoretical spatial resolution expressed by Eq. (1). Although controlled experimental results presented in this study using high power laser and non-scattering media successfully proved the concept, for this method to be fully standardized and validated for biological samples or tissue simulating phantoms, a quantitative discrimination method of signal peaks will be studied and suggested in future works.
(DHS, SHR, CGS) This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011–0030075, 2011–0030781). This research was financially supported by the Ministry of Knowledge Economy(MKE), Korea Institute for Advancement of Technology(KIAT) and Gangwon Leading Industry Office through the Leading Industry Development for Economic Region.
(DHK) Disclaimer: The mention of commercial products, their sources, or their use in connection with material reported herein is not to be construed as either an actual or implied endorsement of such products by the Department of Health and Human Services.
- Xu M, Wang LV: Photoacoustic imaging in biomedicine. Rev Sci Instrumen 2006, 77: 041101. 10.1063/1.2195024View ArticleGoogle Scholar
- Wang X, Pang Y, Ku G, Stoica G, Wang LV: Three-dimentional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact. Opt Lett 2003, 28: 1739-1741. 10.1364/OL.28.001739View ArticleGoogle Scholar
- Zemp RJ, Song L, Bitton R, Shung KK, Wang LV: Realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer. Opt Express 2008, 16: 7915-7928. 10.1364/OE.16.007915View ArticleGoogle Scholar
- Maslov K, Zhang HF, Hu S, Wang LV: Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries. Opt Lett 2008, 33: 929-931. 10.1364/OL.33.000929View ArticleGoogle Scholar
- Li X, Xi L, Jiang R, Yao L, Jiang H: Integrated diffuse optical tomography and photoacoustic tomography: phantom validations. Biomed Opt Express 2011, 2: 2348-2353. 10.1364/BOE.2.002348View ArticleGoogle Scholar
- Cook JR, Bouchard RR, Emelianov SY: Tissue-mimicking phantoms for photoacoustic and ultrasonic imaging. Biomed Opt Express 2011, 2: 3193-3206. 10.1364/BOE.2.003193View ArticleGoogle Scholar
- Wang X, Xu Y, Xu M, Yokoo S, Fry ES, Wang LV: Photoacoustic tomography of biological tissues with high cross-section resolution: reconstruction and experiment. Med Phys 2002, 29: 2799-2805. 10.1118/1.1521720View ArticleGoogle Scholar
- Yuan Z, Zhang Q, Jiang H: Simultaneous reconstruction of acoustic and optical properties of heterogeneous media by quantitative photoacoustic tomography. Opt Express 2006, 14: 6749-6754. 10.1364/OE.14.006749View ArticleGoogle Scholar
- Dean-Ben XL, Ma R, Razansky D, Ntziachristos V: Statistical approach for optoacoustic image reconstruction in the presence of strong acoustic heterogeneities. IEEE Trans Medical Imaging 2011, 30: 401-408.View ArticleGoogle Scholar
- Davies SJ, Edwards C, Taylor GS, Palmer SB: Laser-generated ultrasound: its properties, mechanisms and multifarious applications. J Phys D: Appl Phys 1993, 26: 329-348. 10.1088/0022-3727/26/3/001View ArticleGoogle Scholar
- Ko SH, Ryu SG, Misra N, Pan H, Grigoropoulos CP, Kladias N, Panides E, Domoto GA: Laser induced short plane acoustic wave focusing in water. Appl Phys Lett 2007, 91: 051128. 10.1063/1.2768192View ArticleGoogle Scholar
- Ko SH, Ryu SG, Misra N, Pan H, Grigoropoulos CP, Kladias N, Panides E, Domoto GA: Laser induced plane acoustic wave generation, propagation, and interaction with rigid structures in water. J Appl Phys 2008, 104: 073104. 10.1063/1.2980014View ArticleGoogle Scholar
- Don-Liyanage DKL, Emmony DC: Schlieren imaging of laser-generated ultrasound. Appl Phys Lett 2001, 79: 3356-3357. 10.1063/1.1418028View ArticleGoogle Scholar
- Wang LV, Yang X: Boundary conditions in photoacoustic tomography and image reconstruction. J Biomed Opt 2007, 12: 014027. 10.1117/1.2709861View ArticleGoogle Scholar
- Andreev VG, Karabutov AA, Araevsky AA: Detection of ultrawide-band ultrasound pulses in optoacoustic tomography. IEEE Trans Ultrasonics Ferroelectrics and Frequency Control 2003, 50: 1383-1390.View ArticleGoogle Scholar
- Madsen EL, Zagzebski JA, Banjavie RA, Jutila RE: Tissue mimicking materials for ultrasound phantoms. Med Phys 1978, 5: 391-394. 10.1118/1.594483View ArticleGoogle Scholar
- Del Grosso VA, Mader CW: Speed of Sound in Pure Water. J Acous Soc America 1972, 52: 1442-1446. 10.1121/1.1913258View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.