 Research
 Open access
 Published:
Quantification of compensatory processes of postnatal hypoxia in newborn piglets applying shortterm nonlinear dynamics analysis
BioMedical Engineering OnLine volumeÂ 10, ArticleÂ number:Â 88 (2011)
Abstract
Background
Newborn mammals suffering from moderate hypoxia during or after birth are able to compensate a transitory lack of oxygen by adapting their vital functions. Exposure to hypoxia leads to an increase in the sympathetic tone causing cardiorespiratory response, peripheral vasoconstriction and vasodilatation in privileged organs like the heart and brain. However, there is only limited information available about the time and intensity changes of the underlying complex processes controlled by the autonomic nervous system.
Methods
In this study an animal model involving seven piglets was used to examine an induced state of circulatory redistribution caused by moderate oxygen deficit. In addition to the main focus on the complex dynamics occurring during sustained normocapnic hypoxia, the development of autonomic regulation after induced reoxygenation had been analysed. For this purpose, we first introduced a new algorithm to prove stationary conditions in shortterm time series. Then we investigated a multitude of indices from heart rate and blood pressure variability and from bivariate interactions, also analysing respiration signals, to quantify the complexity of vegetative oscillations influenced by hypoxia.
Results
The results demonstrated that normocapnic hypoxia causes an initial increase in cardiovascular complexity and variability, which decreases during moderate hypoxia lasting one hour (p < 0.004). After reoxygenation, cardiovascular complexity parameters returned to prehypoxic values (p < 0.003), however not respiratoryrelated complexity parameters.
Conclusions
In conclusion, indices from linear and nonlinear dynamics reflect considerable temporal changes of complexity in autonomous cardiorespiratory regulation due to normocapnic hypoxia shortly after birth. These findings might be suitable for noninvasive clinical monitoring of hypoxiainduced changes of autonomic regulation in newborn humans.
Background
The adaptation to extrauterine life, also referred to as transition, involves functional modifications in virtually every organ and bodily system. The most crucial event is the conversion of the fluidfilled lungs into a hollow organ distended with air and capable of gaseous exchange sufficient to warrant extrauterine life. Although the majority of newly born humans establish normal respiratory and circulatory function, 12% may run into difficulties due to a disturbance to the normal adaptive processes required for a smooth transition from intrauterine to extrauterine life [1]. Under such circumstances, sustained systemic hypoxia (for review see [2]) is a common consequence and may lead to fatality or severe lifelong disabilities in case of a progressive lack of oxygen. However, moderate systemic hypoxia of a lower degree is compensated by the neonate during a prolonged time period [3]. This is due to a wellestablished and coordinated neuroendocrine response to systemic hypoxia which functions at birth and is controlled mainly by complex autonomic processing. However, there is a lack of knowledge about the time and intensityrelated alterations of autonomic activity in cardiorespiratory control under hypoxic conditions during the newborn's first period of life. The newborn piglet achieves a degree of maturity at birth similar to that of humans with regard to cardiovascular regulation [4]. Therefore, this species have been considered to be an excellent subprimate laboratory model for comparison studies with human infants. Previous studies using a swine model have shown that hypoxia causes alterations in the autonomic system. Zwiener [5] investigated deterministicchaotic properties that changed after exposure to hypoxia. Gootman [3] determined that hypoxia leads to increased sympathetic activity and to augmented respiratory modulation, but that vagal innervations are inhibited. Evaluations of heart rate variability changes were performed by Sica & Zhao [6] with a focus on linear methods as spectral features. In these studies mainly linear measures from time and frequency domains [3, 6â€“9] were applied.
Nonlinear methods were considered only in a few studies. Zwiener [5] investigated hypoxia, analysing signals from the autonomic nerve system using the correlation dimension, Lyapunov exponent and phase space plots. However, these classical nonlinear methods suffer from the dimensionality curse and require a large number of data points in the time series to reliably estimate the nonlinear features. Unfortunately, this prevents the analysis of shortterm time series and the interactions between different signals due to the complex autonomic regulation. On the other hand nonlinear measures based on entropy estimation as Approximate Entropy (ApEn) or Sample Entropy (SampEn) were able to quantify heart rate irregularity with short segments during episodes of mechanical ventilation and acute anoxia in rats[10]. Though, to determine the nonlinear interplay of various physiological control loops, a multivariate approach based on a combination of different linear and nonlinear parameters is required [11, 12]. Shortterm dynamics due to sustained hypoxia have not been investigated in very young spontaneously breathing piglets thus far. Further, there is only limited information about the complex interactions between cardiovascular and respiratory signals that are altered during the dynamic development from normoxia to hypoxia and subsequently during reoxygenation.
The aim of this study was to characterize physiological states before, during and after hypoxia exposure to spontaneouslybreathing piglets, contributing to the understanding of compensation processes in newborns due to postnatal hypoxia applying shortterm nonlinear dynamics analysis. Moreover, the temporal and complex development of the autonomic regulation had to be explicitly considered between the phases inside states according to normoxia, hypoxia and reoxygenation. For this purpose, a new algorithm for testing stationarity in a shortterm time series was introduced. Linear and especially nonlinear methods were then applied to stationary time series (cardiovascular and respiratory) to investigate the complexity of physiological dynamics inherent in the autonomous system [13]. Several of these nonlinear dynamics have been proven to be of diagnostic relevance or have contributed to risk stratification [12]. We hypothesize that linear and especially nonlinear shortterm indices, when applied to cardiovascular and respiratory signals, can reveal alterations and changes in complexity during the autonomic regulation process of piglets due to their adaptation to hypoxia. Hence, a greater comprehension of temporal and intensity characteristics of autonomic tone might be possible with regard to the adaptive capacity of newborns during a reduced and reestablished oxygen supply. In addition, findings from this study could be useful in the paediatric field for the purpose of recognizing and monitoring hypoxic effects in human newborns.
Methods
Animals and experimental procedure
Animal data were used from seven piglets enrolled in a previous study to estimate the effect of artificial ventilation on regional blood flow and on cardiovascular regulation in newborns [14]. The study was approved by the Committee of the Thuringian State Government for Animal Research. The animals were managed in accordance with the guidelines of the American Physiological Society. In the previous study these seven newborn piglets (23 days old, 1.71 Â± 0.15 kg b.w.) served as a reference group, breathing spontaneously under normoxia and normocapnic hypoxia. They were initially anesthetized with 2.5% isoflurane in nitrous oxide and were given oxygen via a mask. They were maintained throughout the surgical procedure using 1.3% isoflurane in order to record cardiorespiratory signals. During the first 60 minutes, animals stayed under resting conditions (normoxia). After monitoring the resting state, the inspired oxygen fraction was reduced from 0.3 to about 0.1 via an appropriate exchange with nitrogen, causing a normocapnic hypoxia for approximately 60 min. Finally the gas mixture was reestablished to record recovery over a time period of 30 min (reoxygenation).
Data acquisition and preprocessing
High resolution ECG (2048 Hz sampling frequency), synchronized continuously blood pressure and respiration signals were recorded from all seven subjects. Recording time varied from 137 to 191 minutes. ECG was measured by standard limb leads using stainless steel needle electrodes (HSE EKA  Puls IC, Hugo Sachs Elektronik K.G., Germany), while respiration waves were recorded by impedance plethysmography using sticking electrodes on both sides of the chest wall (CardioRespiratorischer Monitor/Apnocard 300, Mechanische WerkstÃ¤tten Radeberg, Germany). Aside, one catheter was advanced through an umbilical artery into the abdominal aorta to record arterial blood pressure (P23Db, Statham Instruments Inc., Hato Rey, Puerto Rico). Physiological parameters were recorded on a multichannel polygraph (MT95K2, AstroMed, W. Warwick, U.S.A.) and stored on harddisk. For analysis, a number of factors that could affect the obtained results via linear and nonlinear methods had to be considered, for example the degree of stationarity, superimposed noise and signal preprocessing (filtering) [12]. Therefore, it became necessary to set up preselection criteria. Due to the bi and multivariate approaches which had been applied in our analyses, it was necessary that adequate processable signals (namely ECG, blood pressure and respiration) with sufficient signal quality and without artefacts were simultaneously available for further processing. Because of the previous study design [14], the aforementioned requirements for signal quality were not present all the time during the investigation. An additional criterion was stationarity due to the fact that some of the applied methods (namely frequency domain analysis and cross conditional entropy) need stationarity conditions.
Stationarity requires that statistical properties such as mean and standard deviation of the investigated time series remain the same throughout the investigated time period. Therefore, we applied a test where the extracted time segments fulfilled our preselection criteria (involving stationarity) in all piglets. Subsequently, the dynamic behaviour before, during and after hypoxia was investigated to analyse the changes in autonomic regulation. For this purpose, two sections, each six minutes in length, were selected from raw data records for the states of hypoxia and reoxygenation (Figure 1). One sixminute phase from the beginning of normoxia (NOR1) was used for subsequent comparison with the early hypoxia (HYP1), late hypoxia (HYP2), early reoxygenation (REOX1) and late reoxygenation (REOX2) phases (Figure 2).
Heart rate time series (tachogram) consisting of successive beattobeat intervals (BBI) as well as systolic (SYS) and diastolic (DIA) blood pressure values were extracted from data records. Then these time series were filtered by applying an adaptive variance estimation algorithm to remove and interpolate ventricular premature beats and artefacts (e.g. movement, electrode noise and extraordinary peaks) to obtain a normaltonormal beat time series (NN) [15]. The phase of respiration was calculated to generate the interval times between consecutive breathing cycles (RESP). For the purpose of interaction analyses all four signals were resampled with a frequency of 4 Hz to obtain synchronised time series.
The extraction of stationary phases
Algorithm:

1.
Dividing the entire BBI time series of NOR, HYP and REOX into two equal parts.

2.
Calculation of the:

a.
mean value meanBBI6 min [ms],

b.
standard deviation sdBBI6 min [ms] and

c.
variation ratio vrBBI6min=\frac{sdBBI6\mathsf{\text{min}}}{meanBBI6\mathsf{\text{mi}}{\mathsf{\text{n}}}^{3}}\left[\mathsf{\text{1}}\xe2\u02c6\u2022\mathsf{\text{m}}{\mathsf{\text{s}}}^{\mathsf{\text{2}}}\right]
for S overlapping subsequences (shift = 1 minute) of 6 minutes window length.

3.
Extracting the phase with the smallest vrBBI6 min.
Test of stationarity
The stationarity of each calculated phase was subsequently controlled using the following algorithm:

1.
Calculations of global mean (global_mean) and global standard deviation (global_std) from the entire time series.

2.
Calculation of two local mean (local_mean) and local standard deviation (local_std) values for the first and second halves of the extracted stationary phase.

3.
Computation of the absolute differences d_mean and d_std between the global and local value each for both mean and std.
d\text{\_}mean\phantom{\rule{2.77695pt}{0ex}}\left(i\right)=abs\left(global\text{\_}meanlocal\text{\_}mean\phantom{\rule{2.77695pt}{0ex}}\left(i\right)\right)(1)d\text{\_}std\phantom{\rule{2.77695pt}{0ex}}\left(i\right)=abs\left(global\text{\_}stdlocal\text{\_}std\phantom{\rule{2.77695pt}{0ex}}\left(i\right)\right);\phantom{\rule{1em}{0ex}}i=1,2(2) 
4.
Determination of the deviation percentage between the two differences for mean and std values respectively to quantify the degree of stationarity of the extracted phase.
dev\text{\_}mean\left[\%\right]=abs\left(1\left(d\text{\_}mean\left(1\right)\xe2\u02c6\u2022d\text{\_}mean\left(2\right)\right)\right)(3)dev\text{\_}std\left[\%\right]=abs\left(1\left(d\text{\_}std\left(1\right)\xe2\u02c6\u2022d\text{\_}std\left(2\right)\right)\right)(4)
The results of stationarity analysis showed that averaged local_mean differed less than 1% while local_std changed to maximally 5%. Due to these marginal deviations, the extracted phases could be evaluated as stationary and were thus used for analysis.
Analysing autonomic regulation
Heart rate variability (HRV) and blood pressure variability (BPV) were quantified by well established indices of time and frequency domains [16] and nonlinear dynamics [12], calculated from univariate cardiovascular and respiratory signals (BBI, SYS, DIA and RESP). For bivariate analyses, wellestablished and validated nonlinear methods, that investigate pairwise interactions between cardiovascular and respiratory signals, were also applied. In addition, all possible combinations of cardiovascular and cardiorespiratory interactions were analysed (i.e. BBISYS, BBIDIA, BBIRESP, SYSDIA, SYSRESP and DIARESP).
Time and frequency domains
Using the time domain, the following standard indices were calculated:

meanNN  the mean value of the NNintervals [ms];

sdNN  standard deviation of the NNintervals [ms].
To estimate the power spectra of the time series, Fast Fourier Transform with a Blackman Harris window function (to avoid leakage effects) was applied to every complete phase. Frequency domain indices were adapted to temporal and spectral features of neonatal cardiovascular signals [17]: very low frequency power (0.005  0.04 Hz), low frequency power (0.04  0.25 Hz) and high frequency power (0.25  0.85 Hz).
Symbolic Dynamics
The analysis of Symbolic Dynamics (SD) has been proven to be adequate for the investigation of complex systems and can describe nonlinear aspects within short and longterm time series [13, 18]. Symbolic dynamics are based upon a coarsegraining of a system's dynamics. The wellestablished algorithm of SD is performed as follows: to classify dynamic changes within the time series they were first transformed into a symbol sequence of four symbols using the alphabet A={0,1,2,3}. Three successive symbols (k = 3; k  word length) were used to characterise symbol strings whereby 64 different word types (bins) were obtained (000, 001, ..., 333) according to the transformation [19]. The resulting histogram contains the probability distribution of each single word type within a word sequence. The following indices from this probability distribution were estimated for BBI, SYS and DIA:

wpsum02  a relative portion (sum/total) of words consisting of only the symbols '0' and '2' to measure decreased HRV [%]

wd_renyi1  Renyi entropy of the word type distribution with Î± = 0.25 as a measure of complexity [bit].
The factor Î± = 0.25 [13] determines the probability distribution p _{ i } of words in the histogram. If 0<Î±, words with small probabilities will mainly determine the value of H _{ renyi } ( Î±).
Joint symbolic dynamics
To characterize the nonlinear interactions of cardiovascular and cardiorespiratory interdependencies the method of Joint Symbolic Dynamics (JSD) was used. This method is based on the analysis of coarsegrained dynamic processes by means of symbols [13, 20, 21]. Here cardiovascular (BBISYS) and cardiorespiratory (BBIRESP) time series were transformed into symbol sequences s of different words according to the transformation rules. Only a short alphabet A={0,1} was used for the symbol sequences whereby increasing values were coded as '1' and decreasing and unchanged values were coded as '0', respectively. Therefore, short patterns (words of length 3) were formed. From each word type (k = 64) the normalized probability occurrences were estimated (JSDJSD64 ) using an 8 Ã— 8 word distribution density matrix (rows  BBI, columns  SYS, RESP, respectively). The advantages of JSD are that it considers all types of interval changes and that a rough assessment of the overall cardiovascular and cardiorespiratory regulation of shortterm interactions is obtained. The following indices were estimated:

BBI_SYS_JSD  normalised probability occurrence of specific word types of heart rate and systolic blood pressure time series [â€°];

BBI_RESP_JSD  normalised probability occurrence of specific word types of heart rate and inspiratory cycle time series [â€°].
The interaction of heart rate time series and diastolic blood pressure time series as well as systolic and diastolic blood pressure time series were not performed due to JSD is not validated for these interactions so far.
PoincarÃ© plot analysis
The PoincarÃ© Plot Analysis (PPA) is based on a technique from nonlinear dynamics and represents the nature of time series fluctuations and can be utilized for determining heart beat dynamics with trends [22, 23]. The PoincarÃ© plots are twodimensional graphical representations of each value of either BBI, SYS, DIA or RESP time series plotted against the subsequent value. The PoincarÃ© plot typically illustrates an elongated cloud of points oriented along the line of identity. For the purpose of graphical illustration, an ellipse based on the shape of the point cloud can be drawn in the plot, whereas the centre of the ellipse represents the mean value. The Poincare' plot is based on the notion of different temporal effects due to changes in the vagal and sympathetic modulation of vegetative parameters, without requirement of stationarity in the time series [24]. In general, three indices are calculated based on the PoincarÃ© plots [22, 25, 26]. In this study we calculated the two standard deviations, namely SD1 (the ellipse's minor axis) measuring shortterm variability, and SD2 (the ellipse's major axis), quantifying longterm variability from the BBI, SYS, DIA and RESP time series.
Segmented PoincarÃ© plot analysis
The Segmented PoincarÃ© Plot Analysis (SPPA) is a quite new enhancement of the PPA. Compared to standard PPA, SPPA avoids linear correlation and analyses nonlinear features of dynamic systems [27, 28]. In addition to the standard PPA the graphical representation is divided into sections of 12 symmetrical columns and rows concentrated around the mean value of the scatter plot. The proximity of the particular sections depends on the indices SD1 and SD2 calculated by the PPA. The determined indices represent the percentage of points in each column and row at the rate of all points (Figure 3).
In our study SPPA was applied to analyse univariate signals as well as the interactions between two signals. To provide an example, we calculated the following index:

BBI_DIA_col5  percentage of points in the fifth column of the PoincarÃ© plot of BBI _{ n }vs. synchronous diastolic blood pressure values (DIA _{ n }).
Cross conditional entropy
Cross Conditional Entropy (CCE) provides quantification of the degree of coupling between two signals [29]. Synchronisation occurs when interactive dynamics between two signals are repetitive. Initially the signals are embedded into multiple dimensions. For each dimension the conditional entropy (CE) modified from the Shannon entropy is calculated.CE is a process of sorting and counting mixed patterns and describes the amount of information included in the sample y when the pattern u is given. Based on CE, one can define the synchronisation index which quantifies the amount of information exchanged between the two signals u and y [29]. The larger the synchronisation index, the more coupled are the two signals. In our study CCE was applied to all possible combinations of cardiovascular and cardiorespiratory interactions. For example, we calculated the index SYS_DIA_SI in order to quantify the coupling between systolic and diastolic blood pressure.
Statistics
The parametricpaired ttest was applied for the statistical evaluation of all univariate differences in linear and nonlinear parameters between the different time segments of the piglet's data. Moderate univariate significances were considered for values of p < 0.05 (*) and high significances for values of p < 0.01 (**). To verify ttest usage, the KolmogorovSmirnovTest (KStest) was performed in advance (normal distribution). In addition, adjustments to statistics were applied via multiple testing using the BonferroniHolm method [30] to confirm the ttest results (#). Descriptive statistics were used to describe the basic features of the data in terms of mean value (mean) and standard deviation (std).
Results
The most significant indices, that showed altered dynamics and changes in the complexity of autonomic regulation during hypoxia until the late reoxygenation phase, have been selected. Consequently, the preselected indices (Table 1) provide the following results. For analysis purposes, indices from methods HRV, SD, PPA and CCE showed high significances in discriminating NOR1 from HYP1 and/or HYP2. Regarding the selected indices from these methods, the statistical tests between NOR1 and REOX groups led to various significant trends. Indices from JSD and SPPA (Figure 4) also revealed high significances for the group tests between NOR1 and REOX1 or REOX2.
Linear dynamics
In this context the value of the HRV index BBI_meanNN decreased from NOR to HYP2 and revealed an increased significance level (p = 0.005). During the process of reoxygenation, the value of BBI_meanNN slightly increased, but remained significantly decreased (p = 0.01) in comparison to NOR1. Another time domain parameter, namely BBI_sdNN, was significantly (p = 0.04) higher within the HYP1 group compared to NOR1. During the process from HYP2 to REOX2, this index showed a transient trend to values similar to NOR1. Regarding BPV analysis, indices showed no significant changes for mean values, but instead an altering progression for blood pressure variance. As a result, the time domain index sdNN increased significantly for both systolic and diastolic blood pressure (SYS: p = 0.05, DIA: p = 0.02) in HYP1 compared to NOR1.
The mean value of the ratio of the powers in the low (LF) and the high (HF) frequency range, LF/HF, from NNintervals was higher in hypoxia than in normoxia and reoxygenation. However these indices revealed no significant differences caused by relatively high std, but LF power tend to increase due to hypoxia.
Nonlinear dynamics
SD complexity measure wpsum02, a measure for decreased HRV, significantly increased (p = 0.005) in HYP2 for BBI. Concerning BPV, the complexity index wd_renyi1 showed high significances for both systolic and diastolic blood pressure for the comparison between NOR1 and HYP1. This parameter lasted high significantly increased for SYS and moderate significantly for DIA during HYP2. During reoxygenation SYS_wd_renyi1 adapted to values similar to NOR1 without any significant differences while DIA_wd_renyi1 maintained significantly (p = 0.05) increased. Shortterm blood pressure fluctuations were found to be high significantly (p = 0.001) increased during both hypoxia states. This was revealed by the PPA index SD1. According to JSD, the significances of index BBI_RESP_JSD revealed an increasing trend (from p = 0.017 to p = 0.003) during reoxygenation. This index describes the percentage of contrary cardiorespiratory interactions which significantly decreased from REOX1 to REOX2. Confirmedly, the univariate SPPA index RESP_row8 showed a high significance (p = 0.004) for group test NOR1 vs. REOX1. On the contrary, the cardiovascular index BBI_SYS_JSD significantly (p = 0.014) declined in HYP1 and HYP2 compared to NOR1. During the investigation of differences caused by hypoxia, significant results were also found using the CCE method. In this regard, the synchronisation index SYS_DIA_SI, describing the coupling quantity of DIA and SYS (synchronisation), increased high significantly (p = 0.009) for both hypoxia states.
Discussion
In this study we analysed the effect of hypoxia on autonomic regulation in newborn piglets by applying linear and nonlinear methods to cardiovascular and respiratory signals. The results indicated that control mechanisms of the autonomic nervous system (ANS) in newborn piglets are influenced considerably by exposure to a hypoxic condition as well as to the inversion to normoxia. The results demonstrated that hypoxic conditions lead to an increase in sympathetic activity which decreases during the hypoxia process. This is in accordance with other studies concerning the analysis of autonomic regulation in piglets exposed to hypoxia [3, 5, 9, 14, 31â€“33]. These findings indicate that hypoxia induces timedependent changes in the cardiovascular and respiratory nervous system. In fact, acute normocapnic hypoxia decreases cardiac vagal tone and increases sympathetic activity resulting in an increased respiration rate and heart rate. After resetting the normoxic supply, cardiovascular and respiratory response diminished as the indices variably changed to conditions similar to those observed before hypoxic exposure.
Regarding linear time domain analyses, HRV index BBI_meanNN showed changes in autonomic regulation due to hypoxia. The adaptation to the restricted oxygen supply proceeded faster and more strongly while the recovery process proceeded in a slower and rather unexpected way. The time domain index BBI_sdNN could only discriminate between NOR1 and HYP1. This result suggests that the piglets regulate with a higher initial variance, but then already regulate to lower variability in terms of adapting to the oxygen deficit. Harris et al. [34] investigated the associations between cardiovascular function and neurological outcome following acute global hypoxia in 20 anaesthetized newborn piglets and found a high degree of variability in cardiovascular function (mean arterial blood pressure and HR) in response to hypoxia. In addition, our study revealed that increased HRV diminished during prolonged hypoxia. This was determined through shortterm analyses from early and late time periods during hypoxia. During reoxygenation, BBI_sdNN showed a stronger trend towards values that conformed to normoxic conditions. By contrast, the BBI_meanNN has not already been regulated to the normoxic level at the end of reoxygenation. Significances revealed by HRV indices could only be confirmed for BBI_meanNN using the BonferroniHolm method. BPV time domain analysis revealed in part similar findings to that of HRV. The adaptation during reoxygenation to normoxia proceeded faster, probably because of the priority of blood pressure regulation. The standard time domain indices SYS_meanNN and DIA_meanNN did not change significantly during the entire investigation. This is in accordance with a study by Gootman [3] showing that no significant changes in blood pressure amplitude are characteristic of premature responses to stimulation of baroreceptors and cardiopulmonary receptors. Moreover, they suggest a direct, hypoxiainduced, stimulation of the sympathetic nervous system (HR increase), overriding any baroreceptor inhibition due to the 10% increase in blood pressure.
In addition indices derived from frequency domain revealed no significances in discriminating groups although a shift of LF and HF components towards higher frequencies was noticed as described by Sica [9], reflecting much higher respiratory and heart rates. Respiration peaks were mostly located at high frequency range between 0.5 and 0.8 Hz associated with vagal control of heart rate [31]. The progress of HRV index LF/HF indicated to a parasympathetic withdrawal and an ongoing sympathetic predominant activation in autonomic tone during hypoxia (Table 1). These influences diminished after reversal to reoxygenation.
The nonlinear HRV index BBI_wpsum02 showed an increased complexity of BBI at the beginning of hypoxia. Therefore the symbolcoded time series of the early hypoxic BBI included more different word types, indicating higher complexity. At the end of the hypoxic state this trend reversed because HRV complexity measure BBI_wpsum02 significantly increased. Hence, a higher number of words including only symbols "0" and "2" implies lower variability and, as a result, less complexity [35]. Likewise, the entropy index wd_renyi1 revealed a higher complexity for both systolic and diastolic blood pressure under hypoxic conditions. An increased complexity in blood pressure was also found by Voss [36] in patients with dilated cardiomyopathy, observed by Shannon entropy. This specific heart disease and normocapnic hypoxia represent risk states for humans and animals that can be recognized via an increased blood pressure complexity. The nonlinear PPA method could support these findings on the basis of increased shortterm variability (SD1). This index was produced clearer results than linear variability measures, although a high correlation between these indices is given.
According to bivariate analysis using nonlinear methods, findings showed that cardiovascular and respiratory signals from newborn piglets imply not only linear but also nonlinear properties which were altered by the autonomous system as a result of normocapnic hypoxia. Results of SPPA, JSD and CCE analyses are in accordance with findings by Zwiener [5], confirming the existence and alterations of linear and nonlinear properties in cardiovascular and respiratory signals recorded from newborn piglets. Analyses from JSD pointed out different results for cardiovascular interactions in contrast to cardiorespiratory interactions. The index BBI_SYS_JSD, determining cardiovascular interactions, decreased significantly during prolonged normocapnic hypoxia. This result could be a reference to less vagal activity due to normocapnic hypoxia. Contrastingly, the index BBI_RESP_JSD, manifesting the extent of vagal modulation between heart and respiratory rates, showed a continuously decreasing trend during reoxygenation. This index was reduced for all hypoxic and recovery periods, which suggests a high BBIRESP coherence and a pronounced respiratory sinus arrhythmia (RSA) after restored oxygen ratio [5].
Significant differences were only affirmed for the cardiorespiratory interaction index during late reoxygenation via multiple testing. Both JSD indices led to the assumption that interactions concerning heart rate are more affected by respiration modulation than by blood pressure fluctuations. Presumably, hypoxic exposure is a severe interference to the newborn piglet whose immature autonomous system, especially with regard to respiration regulation, needs time to adapt to recovered oxygen fraction. SPPA indices discriminated groups involving both univariate and bivariate analyses. Percentages of data points related to specific columns and rows in the PoincarÃ© plot were thus either significantly increased or decreased during normocapnic hypoxia. One noticeable SPPA index was BBI_DIA_col5. This index revealed an increase in point percentage caused by hypoxia which showed ratios equal to values for normoxic conditions already in the late hypoxia stage and following periods. The univariate SPPA index RESP_row8 could contribute to the results of cardiorespiratory analyses in that the JSD findings reflect ongoing respiratory regulatory processes other than those found in normoxia. Nonlinear dynamics to investigate the synchronisation of two signals were applied using the CCE method, calculating entropies as measures of complexity. Regarding CCE, the blood pressure time series revealed a highly significant synchronisation index SYS_DIA_SI for HYP1 and HYP2, suggesting a high predictability level for DIA when SYS is given. The potential reason for the higher blood pressure synchronisation could be the augmented peripheral vasoconstriction, due to increased sympathetic activity during normocapnic hypoxia [34]. After initialising recovery, the synchronisation index decreased in the direction of normoxic values.
A limitation of this study is the small sample number of analysed newborn piglets (N = 7). In experimental animal studies, however, it is usual to investigate such "smallnumbered" samples, especially in the case of newborns. Furthermore, the large amount of determined indices (n>100) from uni and bivariate signal analyses in combination with the small sample size could influence the results of statistical significance tests. Therefore, a statistical adjustment was performed in addition by multiple testing using the BonferroniHolm method [30] to confirm the ttest results.
Conclusions
In conclusion, findings from linear and nonlinear dynamics confirmed the initially higher complexity and higher degree of interactions between cardiovascular and respiratory signals under increased sympathetic influence [2, 6, 37] due to normocapnic hypoxia. Ongoing normocapnic hypoxia leads to a gradual decrease in cardiovascular variability and complexity. Thus, a moderate adaptation of the autonomous system due to the restricted oxygen supply during late normocapnic hypoxia is already present. During reoxygenation, regulating mechanisms related to ventilation are prolonged and ongoing, presumably due to an unstabilised blood acidbase balance [38â€“40]. By contrast, the adaptation of HRV, BPV and cardiovascular interactions proceed much faster and seem to play a superior role in the autonomous regulatory process. To reveal these findings, it is necessary to analyse more than one shortterm section (dynamical behaviour) of noninvasive parameters during prolonged hypoxia and reoxygenation. Furthermore, extensive blood gas analyses could be reduced in the case of noninvasive monitoring of newborns suffering from hypoxia. In a further study, the application of appropriate methods to nonstationary transition phases of this animal experiment will be investigated. Investigations of data from healthy premature infants and from neonates with epilepsy are intended to validate the application of this method to humans. To conclude, the results of this study could contribute to the understanding of the compensatory processes of the newborn autonomous system due to postnatal hypoxia and reoxygenation.
Abbreviations
 ANS:

Autonomic nervous system
 BBI:

BeattoBeat Interval
 BPV:

Blood Pressure Variability
 CCE:

Cross Conditional Entropy
 DIA:

Diastolic blood pressure
 ECG:

Electrocardiogram
 HRV:

Heart Rate Variability
 JSD:

Joint Symbolic Dynamics
 NN:

NormaltoNormal beat interval
 PPA:

PoincarÃ© Plot Analysis
 SD:

Symbolic Dynamics
 SI:

Synchronisation Index
 Std:

Standard deviation
 SYS:

Systolic blood pressure
 RSA:

Respiratory Sinus Arrhythmia.
References
Sinha SK, Donn SM: Fetaltoneonatal maladaptation. Semin Fetal Neonatal Med 2006, 11: 166â€“173. 10.1016/j.siny.2006.01.008
Bauer R: Stupor and coma: pathophysiology of hypoxiaontogenetic aspects. Suppl Clin Neurophysiol 2004, 57: 681â€“687.
Gootman PM, Gootman N: Postnatal changes in cardiovascular regulation during hypoxia. Adv Exp Med Biol 2000, 475: 539â€“548.
Gootman PM, Gootman N, Buckley BJ: Maturation of central autonomic control of the circulation. Fed Proc 1983, 42: 1648â€“1655.
Zwiener U, Hoyer D, Bauer R, Luthke B, Walter B, Schmidt K, Hallmeyer S, Kratzsch B, Eiselt M: Deterministicchaotic and periodic properties of heart rate and arterial pressure fluctuations and their mediation in piglets. Cardiovasc Res 1996, 31: 455â€“465.
Sica AL, Zhao N: Heart rate variability in conscious neonatal swine: spectral features and responses to shortterm intermittent hypoxia. BMC Physiol 2006, 6: 5. 10.1186/1472679365
Gootman PM, Gandhi MR, Coren CV, Kaplan NM, Pisana FM, Buckley BJ, Armour JA, Gootman N: Cardiac responses elicited by stimulation of loci within stellate ganglia of developing swine. J Auton Nerv Syst 1992, 38: 191â€“200. 10.1016/01651838(92)90030K
Gootman PM, Hundley BW, Sica AL: The presence of coherence in sympathetic and phrenic activities in a developing mammal. Acta Neurobiol Exp (Wars) 1996, 56: 137â€“145.
Sica AL, Ruggiero DA, Zhao N, Gootman PM: Developmental changes in heart rate variability during exposure to prolonged hypercapnia in piglets. Auton Neurosci 2002, 100: 41â€“49. 10.1016/S15660702(02)001443
Goncalves H, HenriquesCoelho T, Bernardes J, Rocha AP, Nogueira A, LeiteMoreira A: Linear and nonlinear heartrate analysis in a rat model of acute anoxia. Physiol Meas 2008, 29: 1133â€“1143. 10.1088/09673334/29/9/010
Cerutti S, Hoyer D, Voss A: Multiscale, multiorgan and multivariate complexity analyses of cardiovascular regulation. Philos Transact A Math Phys Eng Sci 2009, 367: 1337â€“1358. 10.1098/rsta.2008.0267
Voss A, Schulz S, Schroeder R, Baumert M, Caminal P: Methods derived from nonlinear dynamics for analysing heart rate variability. Philos Transact A Math Phys Eng Sci 2009, 367: 277â€“296. 10.1098/rsta.2008.0232
Voss A, Kurths J, Kleiner HJ, Witt A, Wessel N, Saparin P, Osterziel KJ, Schurath R, Dietz R: The application of methods of nonlinear dynamics for the improved and predictive recognition of patients threatened by sudden cardiac death. Cardiovasc Res 1996, 31: 419â€“433.
Zwiener U, Bauer R: Marked reduction of brainstem blood flow in artificially ventilated newborn piglets during normoxia and normocapnic hypoxia. Intensive Care Med 2003, 29: 2277â€“2284. 10.1007/s0013400320075
Wessel N, Voss A: Nonlinear analysis of complex phenomena in cardiological data. Herzschr Elektrophys 2000, 11: 159â€“173. 10.1007/s003990070035
Heart rate variability. Standards of measurement, physiological interpretation, and clinical use. Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology Eur Heart J Task Force 1996, 17: 354â€“381.
Lasky RE, Parikh NA, Williams AL, Padhye NS, Shankaran S: Changes in the PQRST intervals and heart rate variability associated with rewarming in two newborns undergoing hypothermia therapy. Neonatology 2009, 96: 93â€“95. 10.1159/000205385
Kurths J, Voss A, Saparin P, Witt A, Kleiner HJ, Wessel N: Quantitative analysis of heart rate variability. Chaos 1995, 5: 88â€“94. 10.1063/1.166090
Voss A, Kurths J, Kleiner HJ, Witt A, Wessel N: Improved analysis of heart rate variability by methods of nonlinear dynamics. J Electrocardiol 1995, 28(Suppl):81â€“88.
Baumert M, Walther T, Hopfe J, Stepan H, Faber R, Voss A: Joint symbolic dynamic analysis of beattobeat interactions of heart rate and systolic blood pressure in normal pregnancy. Med Biol Eng Comput 2002, 40: 241â€“245. 10.1007/BF02348131
Caminal P, Giraldo BF, Vallverdu M, Benito S, Schroeder R, Voss A: Symbolic dynamic analysis of relations between cardiac and breathing cycles in patients on weaning trials. Ann Biomed Eng 2010, 38: 2542â€“2552. 10.1007/s1043901000271
Kamen PW, Tonkin AM: Application of the Poincare plot to heart rate variability: a new measure of functional status in heart failure. Aust N Z J Med 1995, 25: 18â€“26. 10.1111/j.14455994.1995.tb00573.x
Weiss JN, Garfinkel A, Spano ML, Ditto WL: Chaos and chaos control in biology. J Clin Invest 1994, 93: 1355â€“1360. 10.1172/JCI117111
Tulppo MP, Makikallio TH, Takala TE, Seppanen T, Huikuri HV: Quantitative beattobeat analysis of heart rate dynamics during exercise. Am J Physiol 1996, 271: H244â€“252.
Brennan M, Palaniswami M, Kamen P: Poincare plot interpretation using a physiological model of HRV based on a network of oscillators. Am J Physiol Heart Circ Physiol 2002, 283: H1873â€“1886.
Huikuri HV, Seppanen T, Koistinen MJ, Airaksinen J, Ikaheimo MJ, Castellanos A, Myerburg RJ: Abnormalities in beattobeat dynamics of heart rate before the spontaneous onset of lifethreatening ventricular tachyarrhythmias in patients with prior myocardial infarction. Circulation 1996, 93: 1836â€“1844.
Voss A, Fischer C, Schroeder R, Figulla HR, GÃ¶rnig M: Segmented PoincarÃ© plot analysis for risk stratification in patients with dilated cardiomyopathy. Methods of Information in Medicine 2010, 49: 511â€“515. 10.3414/ME09020050
Seeck A, Baumert M, Fischer C, Khandoker A, Faber R, Voss A: Advanced Poincar'e plot analysis differentiates between hypertensive pregnancy disorders. Physiol Meas 2011, in press.
Porta A, Baselli G, Lombardi F, Montano N, Malliani A, Cerutti S: Conditional entropy approach for the evaluation of the coupling strength. Biol Cybern 1999, 81: 119â€“129. 10.1007/s004220050549
Hemmelmann C, Ziegler A, Guiard V, Weiss S, Walther M, Vollandt R: Multiple test procedures using an upper bound of the number of true hypotheses and their use for evaluating highdimensional EEG data. J Neurosci Methods 2008, 170: 158â€“164. 10.1016/j.jneumeth.2007.12.013
Lipsitz LA, Pincus SM, Morin RJ, Tong S, Eberle LP, Gootman PM: Preliminary evidence for the evolution in complexity of heart rate dynamics during autonomic maturation in neonatal swine. J Auton Nerv Syst 1997, 65: 1â€“9. 10.1016/S01651838(97)000283
Mohan RM, Golding S, Paterson DJ: Intermittent hypoxia modulates nNOS expression and heart rate response to sympathetic nerve stimulation. Am J Physiol Heart Circ Physiol 2001, 281: H132â€“138.
Zhao N, Khan M, Ingenito S, Sica AL, Gootman N, Gootman PM: Electrocardiographic changes during postnatal development in conscious swine with cardiac autonomic imbalance. Auton Neurosci 2001, 88: 167â€“174. 10.1016/S15660702(01)002363
Harris TA, Healy GN, Colditz PB, Lingwood BE: Associations between serum cortisol, cardiovascular function and neurological outcome following acute global hypoxia in the newborn piglet. Stress 2009, 12: 294â€“304. 10.1080/10253890802372414
Boettger MK, Weber K, Grossmann D, Gajda M, Bauer R, Bar KJ, Schulz S, Voss A, Geis C, Brauer R, Schaible HG: Spinal tumor necrosis factor alpha neutralization reduces peripheral inflammation and hyperalgesia and suppresses autonomic responses in experimental arthritis: a role for spinal tumor necrosis factor alpha during induction and maintenance of peripheral inflammation. Arthritis Rheum 2010, 62: 1308â€“1318.
Voss A, Schroeder R, Truebner S, Goernig M, Figulla HR, Schirdewan A: Comparison of nonlinear methods symbolic dynamics, detrended fluctuation, and Poincare plot analysis in risk stratification in patients with dilated cardiomyopathy. Chaos 2007, 17: 015120. 10.1063/1.2404633
Aouad LJ, Tam K, Waters KA: Effects of acute intermittent hypercapnic hypoxia on insulin sensitivity in piglets using euglycemic clamp. Metabolism 2008, 57: 1056â€“1063. 10.1016/j.metabol.2008.03.008
Cheung PY, Obaid L, Emara M, Brierley Y, Johnson ST, Chan GS, Jewell L, Korbutt G, Bigam DL: Cardiorenal recovery of hypoxic newborn pigs after 18%, 21% and 100% reoxygenation. Intensive Care Med 2008, 34: 1114â€“1121. 10.1007/s0013400810089
Solas AB, Kalous P, Saugstad OD: Reoxygenation with 100 or 21% oxygen after cerebral hypoxemiaischemiahypercapnia in newborn piglets. Biol Neonate 2004, 85: 105â€“111. 10.1159/000074966
Tichauer KM, Brown DW, Hadway J, Lee TY, St Lawrence K: Nearinfrared spectroscopy measurements of cerebral blood flow and oxygen consumption following hypoxiaischemia in newborn piglets. J Appl Physiol 2006, 100: 850â€“857.
Acknowledgements
This study was partly supported by grants from Deutsche Forschungsgemeinschaft (DFGVO 505/81 and DFGVO 505/82).
Author information
Authors and Affiliations
Corresponding author
Additional information
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
BW and RB conducted the animal experiment and collected and assembled the data. SS and SR drafted the article and analysed and interpreted the data. ME, KS, HW and RB are wellversed in newborn physiology and participated in manuscript revision. AV conceived the study and critically revised it for significant intellectual content. All authors gave final approval of the version to be published.
Authorsâ€™ original submitted files for images
Below are the links to the authorsâ€™ original submitted files for images.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Schulz, S., Reulecke, S., Eiselt, M. et al. Quantification of compensatory processes of postnatal hypoxia in newborn piglets applying shortterm nonlinear dynamics analysis. BioMed Eng OnLine 10, 88 (2011). https://doi.org/10.1186/1475925X1088
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/1475925X1088