Fig. 2

The comparison of peri-implant bone ECM and PIEZO1 expression between the compressive and tensile sides of peri-implant bone. a Peri-implant bone ECM was observed by VG staining in the compressive and tensile sides. Bone ECM (orange), the implant (black). Scale bar, 100 μm. b The bone ECM-to-implant contact (%) and the peri-implant bone ECM area (%) were quantified at ROIs in the compressive and tensile sides (n = 9, Mann–Whitney test). c Immunofluorescence staining of PIEZO1 in the compressive and tensile sides. PIEZO1 (green), DAPI (blue). Scale bar, 500 μm. d Qualification of intensity and area fraction of positive PIEZO1 cells at ROIs in the compressive and tensile sides (n = 9, Mann–Whitney test). All data are represented as mean ± SD. ***p < 0.001. NS no significance