Table 1 Genes and primers for monitoring osteodifferentiation
From: Human cells with osteogenic potential in bone tissue research
Gene | Forward sequence | Reverse sequence |
|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)—housekeeping | GAGTCCACTGGCGTCTTCAC | GTTCACACCCATGACGAACA |
Runt-related transcription factor 2 (Runx2) | GTAGATGGACCTCGGGAACC | GAGCTGGTCAGAACAAAC |
Osterix (Osx, Sp7) | TTCTGCGGCAAGAGGTTCACTC | GTGTTTGCTCAGGTGGTCGCTT |
Alkaline phosphatase (ALP) | GGAACTCCTGACCCTTGACC | TCCTGTTCAGCTCGTACTGC |
Collagen type I (Col1) | GAGTGCTGTCCCGTCTGC | TTTCTTGTTCGGTGGGTG |
Bone sialoprotein (BSP) | GGCAGTAGTGACTCATCCGAAG | GAAAGTGTGGTATTCTCAGCCTC |
Osteopontin (OPN) | GTTTCTCAGACCTGACATCC | CATTCAACTCCTCGCTTTCC |
Osteonectin (SPARC, BM-40) | TGCCTGATGAGACAGAGGTGGT | CTTCGGTTTCCTCTGCACCATC |
Osteocalcin (OCN) | GGCAGCGAGGTAGTGAAGAG | CTCACACACCTCCCTCCTG |
Fibroblast growth factor-23 (FGF-23) | GGAACAGCTACCACCTGCAGAT | CACCACAAAGCCAGCATCCTCT |
Bone morphogenetic protein-2 (BMP2) | CAGACCACCGGTTGGAGA | CCACTCGTTTCTGGTAGTTCTTC |
Receptor activator of nuclear factor κB ligand (RANKL) | GCCTTTCAAGGAGCTGTGCAAAA | GAGCAAAAGGCTGAGCTTCAAGC |
Osteoprotegerin (OPG) | GGTCTCCTGCTAACTCAGAAAGG | CAGCAAACCTGAAGAATGCCTCC |
Sclerostin (SOST) | GGAGCTGGAGAACAACAAGACC | TCACGTAGCGGGTGAAGTGCAG |