Fig. 2

VLS tissues and normal tissues directly differentially expressed mRNA. A Volcanic map of VLS differential gene expression level. The x axis represented the normalized difference (VLS group/normal group). The y axis represented the normalized P value. In the figure, red represented the up-regulated significantly differentially expressed genes, blue represented the down-regulated significantly differentially expressed genes, and the gray dots represented the non-significantly differentially expressed genes. B Statistical chart of down-regulated frequency of VLS genes with significantly different expression. The red column represented up-regulated gene frequency, and the blue column represented down-regulated gene frequency. C Cluster analysis diagram of VLS differential gene expression level. The horizontal axis was the sample (VLS group/normal group), and the vertical axis is the gene. Different colors represent different gene expression levels, and the color ranges from blue to white to red to indicate the expression amount (log10(FPKM + 1)) from low to high. High expression genes were in red and low expression genes were in dark blue