Fig. 7

The regulation of SIRT1 inhibitor EX527 on the inflammation and oxidative stress of LPS-induced A549 cells treated with wogonin. The mRNA expression levels of A TNF-α, B IL-6 and C IL-1β were detected by RT-qPCR in LPS-induced A549 cells treated with wogonin and EX527. ELISA kits were used to evaluate the concentrations of D TNF-α, E IL-1β and F IL-6 in LPS-induced A549 cells treated with wogonin and EX527. G The expression of inflammation-related proteins was examined with western blot analysis in LPS-induced A549 cells treated with wogonin and EX527. The activities of H SOD, I GSH-Px and the levels of J MDA, K ROS were evaluated by kits in LPS-induced A549 cells treated with wogonin and EX527. Data were obtained from three independent experiments (n = 3). ***P < 0.001 vs. control; ^###P < 0.001 vs. LPS; ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 vs. LPS + wogonin