Table 2 The relation between the electrophoretic movement of pDNA (LE), measured %TR, electric pulse energy given in terms of U2 × tE (also proportional to Joule heating QJoule) and electromobilities μ (obtained from [36] for a less dense gel (0.35% w/w collagen) and more dense gel (3% collagen) for different length of the electric pulses: 8 × 200 μs, 8 × 1 ms, 8 × 5 ms, 8 × 10 ms; the applied electric field E = 0.8 kV/cm
From: The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model
Pulse parameters E = 0.8 kV/cm | Electromobility μ 0.35% gel (m2/Vs) | Electromobility μ 3% gel (m2/Vs) | LE 0.35% collagen (μm) | LE 3% collagen (μm) | U2 × tE (V2s) | %TR (0.35% collagen) |
|---|---|---|---|---|---|---|
8 × 200 μs | 0.2 × 10–8 | 0.03 × 10–8 | 0.256 | 0.0384 | 40.96 | 0.65 |
8 × 1 ms | 1 × 10–8 | 0.03 × 10–8 | 6. 4 | 0.192 | 204.8 | 1.55 |
8 × 5 ms | 2.4 × 10–8 | 0.03 × 10–8 | 76.8 | 0.96 | 1024 | 3.58 |
8 × 10 ms | 2.8 × 10–8 | 0.03 × 10–8 | 179.2 | 1.92 | 2048 | 2.16 |