Fig. 5
From: The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model
A Diffusion of pDNA into 3D collagen gel, where pDNA was applied on the top of the 3D gel. The distribution profile c (z, tinc) is calculated from Eq. (4), where z is the distance from the top of 3D gel and tinc is the time of pDNA application on the top of the gel, the diffusion constant is: D = 3 × 10–8/cm2. B Comparison of the theoretically calculated % electrotransfection (%TR) based on the diffusion model of pDNA in 3D gel (Eqs. 4–6) and experimental values (exp) for different times tinc. Initial pDNA concentration applied on the top of the gel was c0 = 90 µg/ml, 8 × 5 ms electric pulses, E = 0.8 kV/cm were applied. Results are presented as a mean ± standard deviation