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Fig. 5 | BioMedical Engineering OnLine

Fig. 5

From: Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

Fig. 5

Interference with BTG2 can partially reverse the effects of interference with CPSF6 on apoptosis and glycolysis of hepatocellular carcinoma cells. A The viability of Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was detected by MTT assay. B The apoptosis of Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was analyzed by TUNEL assay. C The expression of apoptosis-related proteins in Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was determined by Western blot analysis. D The glucose uptake activity of Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was analyzed by glucose assay kit. E The lactate production of Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was analyzed by lactate assay kit. F HK-2 expression in Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was determined by Western blot analysis. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. Control group. ###P < 0.001 vs. sh-NC group. $P < 0.05, $$P < 0.01 and $$$P < 0.001 vs. sh-CPSF6-2 group. N = 3

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BioMedical Engineering OnLine

ISSN: 1475-925X

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