From: Processing of collagen based biomaterials and the resulting materials properties
Unwanted adsorptive | Used agent | Removal mechanism |
---|---|---|
Monovalent cations | Acids (H+) | Ion exchange |
Divalent cations | Acids (H+) | Ion exchange |
Chelating agents | Sequestration | |
Monovalent anions | Alkaline; chloride | Ion exchange |
Saccharides | Alkaline treatment | Degradation |
Enzymes | Degradation | |
Proteins | Proteases | Degradation |
Solvent | Exchange | |
Surfactants (Triton X 100; CHAPS) | Exchange and dissolving | |
Lipophilic components | Surfactants (SDS; Triton X 100) | Dissolving |
Solvent | Dissolving | |
Nucleic acids | Nucleases | Degradation |
Endotoxines | Peroxide, alkaline, acid | Unknown |