Table 10 Impurities, agents to remove them and assumed reaction mechanisms
From: Processing of collagen based biomaterials and the resulting materials properties
Unwanted adsorptive | Used agent | Removal mechanism |
|---|---|---|
Monovalent cations | Acids (H+) | Ion exchange |
Divalent cations | Acids (H+) | Ion exchange |
| Â | Chelating agents | Sequestration |
Monovalent anions | Alkaline; chloride | Ion exchange |
Saccharides | Alkaline treatment | Degradation |
| Â | Enzymes | Degradation |
Proteins | Proteases | Degradation |
| Â | Solvent | Exchange |
| Â | Surfactants (Triton X 100; CHAPS) | Exchange and dissolving |
Lipophilic components | Surfactants (SDS; Triton X 100) | Dissolving |
| Â | Solvent | Dissolving |
Nucleic acids | Nucleases | Degradation |
Endotoxines | Peroxide, alkaline, acid | Unknown |