Table 1 Maxquant software parameters
Item | Value |
|---|---|
Enzyme | Trypsin |
Max missed cleavages | 2 |
Max missed cleavages | 2 |
Main search | 6Â ppm |
First search | 20Â ppm |
MS/MS tolerance | 20Â ppm |
Fixed modifications | Carbamidomethyl (C) |
Variable modifications | Oxidation (M), acetyl (protein N-term) |
Database | See the project report |
Database pattern | Reverse |
Peptide FDR | ≤ 0.01 |
Protein FDR | ≤ 0.01 |
Time window (match between runs) | 2Â min |
Protein quantification | Razor and unique peptides were used for protein quantification |
LFQ [8] | True |
LFQ min. ratio count | 1 |