Table 2 Primary and secondary antibodies for immunofluorescence staining

I Ab: Mouse-anti-human ZO-1

1:250

Life Technologies, Carlsbad, USA

I Ab: Ki67

1:500

Millipore, Darmstadt, Germany

I Ab: Laminin

1:100

Abcam, Cambridge, UK

I Ab: ColI

1:250

Abcam, Cambridge, UK

II Ab: Alexa Fluor 488 donkey-anti-rabbit

1:800

Molecular Probes, Leicestershire, UK

II Ab: Alexa Fluor 568 donkey-anti-mouse

1:800

Molecular Probes, Leicestershire, UK