Table 1 Reverse-transcriptase-PCR primer sequences, product lengths (bp) and used annealing temperatures
Gene | Forward | Reverse | bp | Tm |
|---|---|---|---|---|
GAPDH | GTTCGACAGTCAGCCGCATC | GGAATTTGCCATGGGTGGA | 229 | 55 |
MITF | AAGTCCTGAGCTTGCCATGT | GGCAGACCTTGGTTTCCATA | 352 | 52 |
BEST | GAATTTGCAGGTGTCCCTGT | ATCAGGAGGACGAGGAGGAT | 214 | 60 |
Tyrosinase | TGC CAA CGA TCC TAT CTT CC | GAC ACA GCA AGC TCA CAA GC | 52 | 316 |