Table 1 Primers used for PCR amplification
From: Automated identification of reference genes based on RNA-seq data
Gene | Direction | Sequence |
|---|---|---|
18S | Forward | 5′-TTT GAT GGT ACC TGC TAC TCG GAT AAC C |
Reverse | 5′-CTC TCC GGA ATC GAA CCC TAA TTC TCC | |
Ubiquitin monomer to pentamer | Forward | 5′-ATGCAGAT(C/T)TTTGTGAAGAC |
Reverse | 5′-ACCACCACG(G/A)AGACGGAG | |
Actin | Forward | 5′-TTG CTC TCG ACT ATG AAC AGG |
Reverse | 5′-CTC TCG GCC CCA ATA GTA ATA | |
Mitogen-activated protein kinase | Forward | 5′-CCAGGCGAGATTTCAGAGAC |
Reverse | 5′-TCGGTTTAAGGTCTCGATGG | |
Proline transporter | Forward | 5′-TTGTAGTGAGGGGCGGTTAC |
Reverse | 5′-CATGCAACCAAAGAAGCAGA | |
l-Ascorbate oxidase homolog | Forward | 5′-ACAAAAGGCATTGCTTGGTC |
Reverse | 5′-GGCCAAAACGAAGTTTACCA | |
Gliceraldehyde-3-phosphate dehydrogenase | Forward | 5′-GGGCAAGATCAAGATTGGAA |
Reverse | 5′-GTCTTCTCGCCGAACAAAAG | |
Salicylic acid-binding protein | Forward | 5′-GCATTGACCCGAAAATCCTA |
Reverse | 5′-AGGATGGCGGATTTGTAGTG | |
S-adenosylmethionine decarboxylase proenzyme | Forward | 5′-AGCTTCTGGCATCAGGAAAA |
Reverse | 5′-AGCCAGTACCCTCTCAAGCA |