Fig. 4

Reconstructed ratio image or “disease map” of a human coronary artery. Difference in fluorescence intensity in normal and atherosclerotic artery samples allowed for discrimination between normal and diseased sites via ratio imaging. A pixel with the calculated AT ratio of ≥2 was depicted by black and a pixel with that of <2 was depicted by white. A pixel located in the area other than artery was depicted by black regardless of AT ratio. Scale bar 4 mm