Figure 2

For each condition considered, the addition of 12.5 dynes/cm² of steady wall shear stress caused a significant decrease in the endothelial cell shape index when compared to static ECs, ***P < 0.001 (two-tailed t test). The addition of 10 μM simvastatin caused a significant increase in the endothelial cell shape index for both non-stimulated and TNF-α stimulated endothelial cells, for both static and flow conditions when compared to the vehicle control condition, ⁺⁺⁺P < 0.001 (one-way analysis of variance with Bonferroni post-tests). Stimulation with 10 ng/mL TNF-α caused a significant decrease in the endothelial cell shape index when compared to non-stimulated endothelial cells for all conditions considered, and for both static and flow conditions, ^{^^^}P < 0.001, ^{^^}P < 0.01 (one-way analysis of variance with Bonferroni post-tests),^>>>P < 0.001 (two-tailed t test). For Sim + TNFa: N = 9; all other conditions: N = 10. These values are reported as the mean ± standard error. Control vehicle control, Sim simvastatin, Mev mevalonate, TNFa TNF-α.