Figure 5
From: Effect of 3D-scaffold formation on differentiation and survival in human neural progenitor cells
Growth and proliferation of ReNcell VM cells in 3D PuraMatrix. (A) 3D culture with 0.5% PM without laminin results in the development of spheroid structures after 5 days of cultivation. (B) Cultivation with 0.5% PM with laminin functionalisation resulted in flat and densely packed cell aggregates. (C) Cultures with 0.25% PM and with laminin functionalisation showed cell aggregates, similar to PML 0.5%, but also loosely composed 3D cellular structures are formed. (D) PML 0.15%, the cellular composition in the matrix is mostly built up of 3D structures.