Figure 3

AFM images of PuraMatrix scaffolds after addition of laminin I. AFM images of PuraMatrix + laminin I scaffolds; scan size: 0.75 × 0.75 μm² (left column) or 4 × 4 μm² (right column). Again, bundles and single PM fibres are observed. In contrast to pure PM scaffolds, bundles are frequent even at 0.15% PM concentration and inhomogeneously distributed over the sample (red circle in Fig. 3B). Between these coarse bundle-networks single PM fibres can be found that are less densely distributed than in the non-functionalised scaffold (green arrows, indicating a reduced surface coverage of PM fibres in comparison to Fig. 2A, C). Hence, the addition of laminin I promotes bundling and decreases the density of isolated PM fibres.