Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: a comparative study in rabbits

Table 1 Scoring system for the histological and immunohistochemical evaluation.

Evaluated parameter		Grade	Score
(A)	Fibrosis	absent	0
		minor	1
		mild	2
		moderate	3
		severe	4
(B)	Tissue cavities	absent	0
		minor	1
		mild	2
		moderate	3
		severe	4
(C)	Necrosis	absent	0
		minor	1
		mild	2
		moderate	3
		severe	4
(D)	Cellular infiltrations:	Cells of each type per field of view:	Score
	• Macrophages	0	0
	• Giant cells	1 to 5	1
	• Heterophil granulocytes	6 to 10	2
	• B-lymphocytes	11 to 15	3
	• T-lymphocytes	16 to 20	4
		>20	5

To determine (A) the grade of fibrosis, i. e. thickness and dimension of the periimplant fibrous capsule, and (B) the amount of tissue cavities within the fibrous tissue, the sections were analysed at a magnification of 50× (eyepiece 5×, lens 10×). A magnification of 400× (eyepiece 40×, lens 10×) was used to evaluate (C) the amount of necrosis and (D) the amount of different cellular infiltrations. Therefore, ten randomly chosen microscopic fields of view, that were located within the periimplant fibrous tissue, were analysed and the mean score value was calculated for each section.

ISSN: 1475-925X