Illustration of the myocardial tissue velocity quantification procedure. (A) Block scheme of the procedure including five steps: Initially. a (ROI) was selected. Subsequently, the ROI was used for cropping the images in the sequence, prior to reduction of the 2D B-mode images’ echo-pattern to 1D projections. The tissue velocity was estimated using spatial phase shift and finally the velocity signal was resampled to increase temporal resolution. (B) Illustration of ROI selection, cropped image and reduction of image to 1D by taking the lateral echogenic maxima. (C) Myocardial velocity was estimated based on the spatial phase shift of the 1D projections between pair-wise consecutive frames. Color of schematic ultrasound images are inverted, where dark area corresponds to an echogenic region.