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Table 1 RT-PCR primers used in the present study

From: A preliminary study for constructing a bioartificial liver device with induced pluripotent stem cell-derived hepatocytes

Gene

Forward Primer

Reverse Primer

Product Size (bp)

AFP

CACTGCTGCAACTCTTCGTA

CTTTGGACCCTCTTCTGTGA

300

Alb

GACAAGGAAAGCTGCCTGAC

TTCTGCAAAGTCAGCATTGG

174

Trf

ACCATGTTGTGGTCTCACGA

ACAGAAGGTCCTTGGTGGTG

135

CPS

AGGATGTCAAGGTGTTTGGC

GCTTAACTAGCAGGCGGATG

 93

β-actin

GACCTCTATGCCAACACAGT

AGTACTTGCGCTCAGGAGGA

139

  1. AFP, α-fetoprotein; Alb, Albumin; Trf, transferrin; CPS, carbamoyl phosphate synthetase.
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