Table 2 Summary of result of different decellularization methods

Triton X-100

 < 0.5 ng/mg

Maintained

Without evaluation

High ECs viability

[30]

SDS

 < 0.5 ng/mg

Maintained

Poor ECs viability

SD

 < 1 ng/mg

Maintained

Poor ECs viability

CHAPS

 < 0.5 ng/mg

Maintained

Poor ECs viability

Enzymatic digestion

 < 0.1%

Intact

Forming a complete, intact ECs layer

[33]

High hydrostatic pressure

small(< 50 ng/mg)

Maintained

Until 15 min

Cell adhesion was similar to TCPS

[16]

Freeze-thawing method

small(< 150 ng/mg)

Maintained

Within 8 min

Cell adhesion decreased during the 7 days cultivation

Perfusion

24.7 ± 1.7 µg/mg

Retained

Without evaluation

Forming a confluent ECs monolayer

[24]