Fig. 7
Effect of concentration of cell seeded on number of cells recovered from cell culture scaffolds for different substrate type and concentration (a), data normalized to Collagen 5 µg/mL, 2.5 × 105 cells/mL (n = 3). Two-way ANOVA, significant effect of concentration of cell seeded (***p = 0.0003), significant difference between concentration seeded for 10 µg/mL fibronectin. Assessment of cell coverage through fluorescent nucleus staining (b), Hoescht 33342, 1 μg/mL, 30 min (bar length = 100 µm, magnification = 40×, Nikon Eclipse 2000 microscope) and through crystal violet staining (c) (bar length = 100 µm, magnification = 40×) for different concentrations of cell seeded