Fig. 4

Stepwise assembly of MTM platform. The paper was flipped and the RGC layer was facing down and contacted with the rMEA as the Step 1. In Step 2, the flow rate of the lower fluid flow was raised to generate a negative pressure that could hold the retinal tissue on the rMEA surface. In Step 3, the sMEA was then added on top of the photoreceptor cells and aligned using a micromanipulator. In Step 4, the upper perfusion chamber was put in place and the assembled MTM platform was placed under the microscope in the electrophysiological rig