Kinematics and temporospatial parameters during gait from inertial motion capture in adults with and without HIV: a validity and reliability study

Background Inertial measurement unit (IMU)-based motion capture systems are gaining popularity for gait analysis outside laboratories. It is important to determine the performance of such systems in specific patient populations. We aimed to validate and determine within-day reliability of an IMU system for measuring lower limb gait kinematics and temporal–spatial parameters (TSP) in people with and without HIV. Methods Gait was recorded in eight adults with HIV (PLHIV) and eight HIV-seronegative participants (SNP), using IMUs and optical motion capture (OMC) simultaneously. Participants performed six gait trials. Fifteen TSP and 28 kinematic angles were extracted. Intraclass correlations (ICC), root-mean-square error (RMSE), mean absolute percentage error and Bland–Altman analyses were used to assess concurrent validity of the IMU system (relative to OMC) separately in PLHIV and SNP. IMU reliability was assessed during within-session retest of trials. ICCs were used to assess relative reliability. Standard error of measurement (SEM) and percentage SEM were used to assess absolute reliability. Results Between-system TSP differences demonstrated acceptable-to-excellent ICCs (0.71–0.99), except for double support time and temporophasic parameters (< 0.60). All TSP demonstrated good mean absolute percentage errors (≤7.40%). For kinematics, ICCs were acceptable to excellent (0.75–1.00) for all but three range of motion (ROM) and four discrete angles. RMSE and bias were 0.0°–4.7° for all but two ROM and 10 discrete angles. In both groups, TSP reliability was acceptable to excellent for relative (ICC 0.75–0.99) (except for one temporal and two temporophasic parameters) and absolute (%SEM 1.58–15.23) values. Reliability trends of IMU-measured kinematics were similar between groups and demonstrated acceptable-to-excellent relative reliability (ICC 0.76–0.99) and clinically acceptable absolute reliability (SEM 0.7°–4.4°) for all but two and three discrete angles, respectively. Both systems demonstrated similar magnitude and directional trends for differences when comparing the gait of PLHIV with that of SNP. Conclusions IMU-based gait analysis is valid and reliable when applied in PLHIV; demonstrating a sufficiently low precision error to be used for clinical interpretation (< 5° for most kinematics; < 20% for TSP). IMU-based gait analysis is sensitive to subtle gait deviations that may occur in PLHIV.


Background
Ever since the first definition of HIV/AIDS in the 1980s, motor impairments were noted and described as defining characteristics of the disease [1]. Unfortunately, motor function remains compromised in people living with HIV (PLHIV) even in the current era of modern antiretroviral therapy (ART) [2]. The HI-virus itself, ART drug toxicity, interactions between various comorbidities, traditional risk factors and synergistic mechanisms to usual aging may all contribute to the observed impairments. Common impairments include muscle weakness and dynapenia [3], peripheral sensory neuropathies [4], motor slowing and postural imbalance associated with white matter alterations [5]. Furthermore, a state of ongoing inflammation or immune activation may cause PLHIV to experience non-AIDS-defining complications resembling geriatric processes (including falls and fractures) at relatively young ages [6].
Indeed, about one-third of young-to middle-aged PLHIV experience falls [7,8] and relatively young PLHIV seem to have walking impairments resembling fall-predisposing gait characteristics in older adults [9]. However, the true gait pattern in PLHIV remains inconclusive, as the gait characteristics that have been assessed are limited to gait speed, cadence and gait initiation time (slowed gait speed being the most consistent observation, while delayed fast gait initiation time and low cadence have been described in PLHIV who are also obese) [9]. Furthermore, these observations remain limited to semiquantitative clinical assessments, meaning that subtle and early impairments in a young population may remain undocumented.
There is currently no evidence from three-dimensional (3D) gait analysis describing the gait patterns of PLHIV. This situation prohibits a sensitive evaluation of movement quality and level of impairment and therefore little understanding of the impact of potential impairments remains. Despite the many parallels that have been drawn between usual aging and the processes associated with HIV disease or treatment (e.g., telomere shortening, increased interleukin-6, reduced bone mineral density), it must be noted that the etiological patterns of chronological aging likely differ from accelerated or accentuated aging due to HIV and/or ART. Different patterns of impairment or functional decline may manifest in younger adults dealing with complex chronic illnesses (such as HIV) and the associated treatment burden compared to the general population of older adults [10]. As the effective, targeted rehabilitation of gait function largely depends on an understanding of the underlying impairments and their interactions, there is a need to more rigorously investigate the gait patterns that may be unique to PLHIV.
Instrumented motion analysis provides 3D data that are accurate and precise. Such quantitative data can more comprehensively describe gait patterns and (even subtle) impairments; supporting clinical decision-making and allowing for early diagnosis and intervention [11]. Although marker-based optical motion capture (OMC) remains the gold standard for human motion capture, inertial motion capture systems are increasingly used for 3D gait analysis in various settings outside of the gait laboratory. Inertial motion capture offers several pragmatic benefits relative to OMC. Such systems are more compact, affordable, portable and user-friendly; making them ideal for use in clinical environments [12]. Inertial motion capture is based on small yet powerful integrated circuits (inertial measurement units or IMUs); typically comprising on-board tri-axial gyroscopes, tri-axial magnetometers and tri-axial accelerometers. Using sensor fusion techniques, the ability of IMUs to accurately track orientation has become advanced [13].
However, IMU output is body-referenced (i.e., absolute skeletal position is not readily available to IMUs), suffers from drifts and ferromagnetic disturbances that need correction [14], and often uses automated processing of measured data to generate time and space parameters (TSP). The user may not be able to interfere in such processing. It is thus important to determine the validity of automatically calculated gait events such as initial contact and toe-off, which are important for determining gait phases and other TSP and for understanding joint motion at specific points and phases of the gait cycle [15].
Although the validity and reliability of IMUs have been investigated in healthy participants and certain patient groups, underlying assumptions and body models may not be the same for other population groups or pathologies [16]. IMU validity and reliability should thus be demonstrated in the condition of intended use [16] to improve the quality of data collection and interpretation. Since the 3D gait patterns of PLHIV have never been reported, it remains unknown which biomechanical impairments they might demonstrate and validation in this population is therefore warranted. In addition, although a recent review [17] reported that IMUs are valid for assessing whole body range of motion (ROM), evidence for reliability is lacking and there is a paucity of studies reporting on comprehensive, clinically relevant gait outcomes [18]. This study therefore aimed to determine the concurrent validity of an IMU system (versus OMC and the Conventional Gait Model as reference standard), and the within-session, between-trial reliability of IMUs for measuring lower limb kinematic and temporospatial gait outcomes in PLHIV and HIV-seronegative participants (SNP). The study further aimed to determine whether a gait analysis conducted using IMUs would differentiate between gait outcomes of PLHIV and SNP in a similar manner to a gait analysis conducted using OMC.

Results
The full set of data for all participants (n = 8 PLHIV and n = 8 SNP) were analyzed for both the IMU and OMC systems (a total of 96 gait trials for each system). IMU-detected gait events (initial contact and toe-off) demonstrated differences from OMC-detected events as follows: initial contact errors for IMUs demonstrated median (interquartile range [IQR]) values of − 10.00 ms (96.25 ms) in SNP and − 7.50 ms (82.50 ms) in PLHIV. Median (IQR) toe-off errors for IMUs were 2.50 ms (95.00 ms) in SNP and − 5.00 ms (62.5 ms) in PLHIV.
Concurrent validity: temporal, spatial, temporophasic and temporospatial parameters (TSP) Between-system differences for TSP demonstrated acceptable-to-excellent intraclass correlation coefficients (ICCs, 0.71-0.99), except for double support time and temporophasic parameters, which demonstrated questionable to poor ICCs (< 0.60) (Fig. 1). All TSP demonstrated good mean absolute percentage errors (≤ 7.40%). RMSE, bias and limits of agreement (LoA) between IMUs and OMC were close to zero for temporal, leg length-normalized spatial and temporospatial parameters in both participant groups. Mean absolute percentage errors were < 2.68% for all parameters except double support time (7.40% and 5.79% for SNP and PLHIV, respectively) and double support percentage (6.96% and 5.69% for SNP and PLHIV, respectively). These latter parameters had the largest mean absolute percentage errors in both groups (Table 1).

Concurrent validity: kinematics
For kinematics, ICCs were acceptable to excellent (0.75-1.00) for all but three ROM and four discrete angles (Fig. 1). RMSE and bias were 0.0°-4.7° for all but two ROM and 10 discrete angles. RMSE, biases and LoA were generally larger in PLHIV (although remaining within 2° from those observed in SNP). Between-system differences were < 5° for all ROM outcomes; except for hip internal rotation (both groups) and hip flexion [in PLHIV: ROM over entire gait cycle (i.e., between two successive occurrences of ipsilateral initial contact) and ROM from pre-swing to initial swing (i.e., from contralateral initial contact to the instant when the ipsilateral swing leg is adjacent to the stance limb)], while angular values at specific time points of the gait cycle tended to exceed 5° (Table 2).

Within-session, between-trial reliability: TSP
In both participant groups, TSP reliability was acceptable to excellent for relative values (ICC 0.75-0.99) (except for stance time and percentage in SNP and single support percentage in both groups) (Fig. 2) as well as for absolute values [percentage standard error of measurement (%SEM) 1.58-15.23]. Spatial parameters showed better absolute reliability in SNP [lower standard error of measurement (SEM), %SEM and upper 95% confidence limit (CL)], temporal and temporophasic parameters were more reliable in PLHIV and temporospatial parameters were more reliable in SNP. However, for all these outcomes, %SEM observed in the two participant groups were within ~ 2% of each other (Table 3).

Within-session, between-trial reliability: kinematic angles
The reliability of IMU-measured kinematic angles was similar between participant groups and demonstrated acceptable-to-excellent relative reliability (ICC 0.76-0.99, except for pelvis rotation at initial contact and peak knee flexion during stance) (Fig. 2).
Clinically acceptable absolute reliability was demonstrated (SEM 0.7°-4.4°) for all but three discrete angles (Table 4). In SNP, pelvis rotation at initial contact and ankle plantarflexion angle at toe-off were the only angles with absolute reliability exceeding 5°, while in PLHIV, peak knee flexion in stance showed an SEM of 5.8°, with an upper 95% CL of 7.0°.

Between-group comparisons (performed separately for IMU and OMC)
Between-group differences are presented here to demonstrate the validity of a clinical gait assessment by both instrumented systems. Selected kinematic gait curves for both groups and systems are presented in Fig. 3 (only sagittal plane traces shown). Directional trends of between-group differences were largely similar in IMU and OMC results for TSP (Table 5) and kinematics angles ( Table 6). For TSP, betweengroup differences and p-values were almost identical for both systems, but less so for temporophasic outcomes. In PLHIV, both OMC and IMUs demonstrated significantly

Discussion
This study assessed the validity and reliability of 3D gait analyses in PLHIV and community-matched SNP using a body-worn IMU system relative to a camera-based OMC system. PLHIV are suggested to suffer subtle gait impairments that may predispose them to adverse functional outcomes. This is the first study to suggest the use of IMUs in PLHIV for measuring a comprehensive set of clinically relevant gait outcomes as a reliable alternative to OMC. In both participant groups, and for most outcomes, the validity (estimated by between-system comparison) and reliability (estimated from repeated testing) of IMU-measured TSP and lower limb angles were deemed acceptable for detecting clinically meaningful differences [20]. In terms of absolute reliability, i.e., measurement error, all 43 gait analysis outcomes were clinically acceptable, except three discrete kinematic angles (pelvis rotation at initial contact, peak knee flexion during stance and ankle plantarflexion at toe-off ). In terms of gait analysis, IMU technology seems sufficiently sensitive to determine gait deviations between PLHIV and SNP.
The IMU and OMC systems demonstrated good agreement, small offsets and acceptable-to-excellent ICCs for all TSP, although less so for double support time and parameters expressed as a percentage of the gait cycle. These findings are similar to those from other validation studies investigating various IMU configurations and reference systems [18,[21][22][23]. In addition, the observed initial contact and toe-off errors of 0.010 s or less are similar to those that have been reported for IMU systems using smaller recording frequencies and different event-detection algorithms [18,24]. Double support time and those parameters expressed as a percentage of the gait cycle (especially double support percentage) showed the largest relative differences and/or worst ICCs in both participant groups. Similar results have been reported recently in a study validating a three-IMU system relative to an instrumented walkway [25]. Errors for double support time and percentage, and single support percentage, tended to be lower in PLHIV relative to SNP. These differences may stem from gait speed differences and/or true pathology [25], although further research is needed to support such speculations. Other IMU validation studies comparing (slower walking) older adults to (faster walking) younger adults have had similar findings for these parameters (lower errors for these outcomes in the older adults) [25,26]. Differences between the IMU and OMC systems were more apparent when comparing (discrete) kinematic angles at specific time points of the gait cycle, and less so when comparing relative joint/segment ROM. This was true for both participant groups. Considering the different technology sources (IMUs versus cameras) as well as models to measure and calculate kinematic angles, these results are not surprising and generally agree with previous studies comparing IMU and OMC technologies [27,28]. Low RMSE and excellent correlations have for example been reported for most lower limb joints during gait when either using the same biomechanical model to calculate angles from IMU segment position data and OMC marker clusters (RMSE below 5°), or after removing the offset between models (RMSE below 9°) [27,29,30]. When however using independent models without offset correction to calculate IMU and OMC kinematics, correlations remained good to excellent while worse RMSE (e.g., up to 28° for the hip) were demonstrated in these studies [27,30]. Our results reaffirm previous observations that discrete angles are not directly comparable between IMU and OMC systems/models, while relative angular ROM seem more comparable [27]. These observations may largely stem from the different ways that segment positions and joint axes definitions are established during the systems' respective calibrations-this would be especially true for the sagittal plane. In addition to between-system differences in segment positions and joint axes definitions, soft tissue artifact may affect marker and IMU positions in different ways; further increasing differences between the systems/models [31].  In both participant groups, TSP demonstrated acceptable-to-excellent reliability (except for three parameters), with measurement errors smaller than what would be considered clinically meaningful. For example, the SEM for gait speed-an outcome commonly measured in clinical function studies in PLHIV [9] -was 0.02 m/s (SEM% < 2%) in both groups, whereas a much larger value of 0.1 m/s has been suggested as being clinically significant [32]. In terms of measurement error for TSP, less reliable results were observed for stance time in SNP and single support percentage in both groups. Potential reasons may be that these outcomes were truly unstable, or an insensitivity of the IMU technology to detect a relatively stable phenomenon. In a study by Washabaugh and colleagues [24], where measurement error that is due to natural walking variations was controlled for by means of treadmill walking, stance and swing percentages were the least reliable TSPs measured by foot-mounted IMUs-suggesting larger instrumentation error for these outcomes.
We found that the trends in reliability of IMU-measured kinematic angles were generally similar between PLHIV and SNP groups and fair-to-excellent for all but three angles (pelvis rotation at initial contact in both groups, ankle plantarflexion at toe-off in PLHIV and peak knee flexion in stance in SNP). The worse findings for these discrete outcomes may suggest that some key events of the gait cycle are inherently more variable in the groups, or that the IMU-and OMC-systems were both more susceptible to, and potentially affected in different ways by, soft tissue artifact at these events; considering artifacts reported for the pelvis (high, up to 25 mm), thigh (high: up to 31 mm) and lateral malleolus (moderate: up to 15 mm) [33]. The relative error of these moderate-to-high soft tissue artifacts will be even larger for motions with small ranges (i.e., low signal-tonoise ratios) [23].
For all other joint/segments and planes, ICC values of between 0.76 and 0.99 were observed, with a relatively small SEM (≤ 4.4°). These results are comparable to published results for within-rater or between-trial reliability for OMC [20] and IMU systems [17,34]. According to a recent systematic review [17], reliability for IMU-measured kinematics across lower limb joints and planes ranged from 0.40 to 0.95 in terms of correlation coefficients and 0.3°-9.9° in terms of absolute errors. However, the authors noted that the small number of studies for each joint did not allow for strong conclusions (e.g., only one study reporting pelvic angles). Most reports of IMU-based systems have not reported the reliability of discrete joint angles; we expand on the existing body of literature in this regard.
In clinical terms, it may be more important to consider absolute rather than relative reliability when interpreting the results for 3D gait analysis-i.e., whether the measurement error renders the instrument meaningful for clinical use [35]. Although the ICC has been widely recommended to assess reliability, it has the disadvantage of being affected by between-participant variability (unlike the SEM). In situations where little variation exists between participants, the ICC will inevitably be low or unmeasurable (as was the case for pelvic obliquity in this study, when calculating an ICC for comparing IMUs and OMC), since it measures the ratio of within-participant variability to between-participant variability [36]. A further example of this limitation is peak knee flexion during stance in SNP, which had a poor ICC of 0.45 but a clinically acceptable SEM of 3.9°. Similarly, the low ICC of pelvis rotation at initial contact was also associated with an acceptable SEM (3.7°) in PLHIV.
When interpreting IMU and OMC data separately to compare gait patterns between PLHIV and SNP, the magnitude and direction of between-group differences (both significant and non-significant) were similar for the two systems. Slowed gait speed-the most consistently reported finding from clinical gait studies in PLHIV [9]-was demonstrated by both systems in terms of clinical but not statistical significance [betweengroup differences of > 0.10 m/s demonstrate by both systems-exceeding the minimum clinically important difference (MCID) reported for usual-paced gait [32]-but p-values for both systems exceeded 0.05]. Both systems also detected significantly increased stance-and double support times in PLHIV (p < 0.05), as well as clinically significantly decreased ankle joint angles in PLHIV (between-group differences exceeding 5°). From a gait analysis point of view, these results support the sensitivity of IMUs to the differences between populations on the level of what an HIV-associated deviation might be. As expected, the kinematic differences between PLHIV and SNP in this relatively young sample were mostly small-although the magnitude of biomechanical differences that would translate into functional limitations in PLHIV remains unknown and an area for future research.

Clinical implications
The validity and reliability of IMU-based gait analyses are not compromised by the presence of HIV. Firstly, this study showed similar trends in validity and reliability in both participant groups. Absolute reliability results indicate a sufficiently low level of measurement error for IMUs to be used for clinical interpretation, and values fall well within the precision error reported for OMC [20]. Secondly, despite the differences in data sources and modeling employed by OMC and IMUs, our results suggest that similar clinical conclusions may be drawn when using either system for clinical gait analysis in PLHIV (e.g., both systems demonstrated the expected kinematic and TSP changes that would logically accompany slow gait in PLHIV relative to SNP). It was not the aim of this paper to describe the deviations potentially occurring in PLHIV, but rather to explore whether an IMU system would be sensitive enough to detect small effects between groups in a similar manner to OMC; indeed this seems to be the case. It should be kept in mind, however, that different IMU systems use different algorithms and because of an inherent offset between IMU-and OMC systems/models, data from IMU and OMC systems should not be used interchangeably.

Limitations
A study limitation is that only usual-paced walking was assessed, and thus study results are not generalizable to very slow or fast speeds. The accuracy of IMUs are reportedly the highest in the range of 1.0-2.2 m/s, and lower at velocities that are either slower or faster than this range [37]. The performance of IMUs when conducting gait analysis in PLHIV should be verified in such speed ranges. Although IMUs proved to be suited to the specific population used in this project, these results may not readily be assumed to hold true in different populations with gross gait pathology or higher BMI. Although OMC served as reference standard, it is susceptible to faulty marker placement [38]; nevertheless, marker-placement by a laboratory-trained physiotherapist likely limited marker placement errors.

Conclusion
To conclude, this study demonstrated valid and reliable results from an IMU system for 3D gait analysis, delivering a wide range of clinically relevant gait outcomes in people with and without HIV. Despite the different data sources and modeling used by IMU and OMC systems, a gait analysis conducted in a unique population, namely PLHIV, provided similar magnitudes and directions of differences relative to healthy individuals, and thus similar clinical conclusions are likely when using either system.

Participants
Eight PLHIV and eight SNP were recruited from a public primary care community health center (

Study design
This study incorporated concurrent validity testing and within-session, between-trial repeated measures reliability testing. Concurrent validity refers to a form of criterion validity where the performance of two different measures is assessed at the same time  19:57 to determine the similarity between the index test/new measure (IMUs in this case) and the criterion measure/reference standard (OMC in this case). Reliability refers to the extent to which repeated measures provide similar results in unchanging individuals [39] and was determined in this study across multiple repeated trials, which all occurred during a single session. A single testing session was thus conducted per participant and a single rater (motion analysis-trained physiotherapist) performed all testing. The study formed part of a larger protocol to study gait features in PLHIV residing in a semi-rural South African setting.

Setting
Data were collected in the Stellenbosch University Central Analytical Facilities (CAF) 3D Human Biomechanics Unit, Tygerberg Medical Campus, Cape Town, South Africa. Participants were transported between the clinic and the motion laboratory using official university transport services.

Sample size
Sample size was based on the SEM (a measure of intra-individual variability) for lower limb kinematic angles across the gait cycle, considering a reported SEM of 4.1° [40]. This was the maximum SEM (hip rotation) reported across tri-planar lower limb angular ROM in healthy adults for usual-paced walking [40]. An MCID of 5° is suggested for lower limb gait kinematics [41]. To establish that a measured SEM of 4.1° is lower than 5° at a one-sided 95% confidence interval (CI), the recommendations by Stratford and Goldsmith [42] were followed. The variance ratio σ 2 s 2 was calculated as 5.0 2 4.1 2 = 1.5 . Using this variance ratio and Table 7 in [42], the required sample size was estimated for a protocol making use of 6 measurements per participant; i.e., a sample size of 9 participants. Sample size was restricted by pragmatic constraints such as participant transportation; thus, a convenience sample of 8 PLHIV and 8 SNP was deemed practical.

Instrumentation and procedures
IMUs and reflective OMC markers of two independent gait analysis systems were fixated simultaneously on the participant (Fig. 4) to collect gait data concurrently using the two systems and their respective biomechanical models.

Inertial measurement unit (IMU) system
The index test was a wireless IMU system (myoMOTION Research Pro, Noraxon USA Inc.) consisting of a receiver and 7 IMUs (for a lower body setup). Each IMU (37.6 mm × 52.0 mm × 18.1 mm; 34 g) has a local coordinate system and measures accelerations and yaw-pitch-roll orientations along three coordinate axes. IMUs were placed on body segments according to a rigid lower body model provided by the IMU system software [myoRESEARCH 3.10.64 (MR3)]. The model considers each body segment as a rigid unit with interlinking joints and assumes a rigid IMU-segment attachment. The system was calibrated before conducting measurements using a neutral standing pose. Gait events (initial contact and toe-off ) were detected using an IMU-based contact detection algorithm provided by the software. The algorithm utilizes gyroscope (foot angular velocity) as well as acceleration measurements from the foot-mounted IMU to identify periods when the foot is in contact with the ground, creating virtual foot contact signals for each foot. A sampling rate of 200 Hz was selected for all IMUs. Our laboratory previously demonstrated the capability of the IMUs to measure angles with a static accuracy of 0.4° ± 0.2° (inclination) and 0.8° ± 0.4° (heading) and dynamic accuracy of 0.9° ± 0.2° (inclination) and 2.0° ± 0.8° (heading). Acquired motion-related signals (IMU data) were transmitted wirelessly by a small radio module to a recording laptop.

Optical motion capture (OMC) system
The reference standard was an OMC system (MX T-series, VICON Motion Systems Limited) and the Plug-in-Gait (PiG) model. The system uses multiple synchronized highresolution, high-speed cameras to reconstruct body posture and provides body segment position (origin) and orientation (axis directions) relative to a global fixed coordinate system. The VICON has previously demonstrated high validity and reliability [43], and the Conventional Gait Model (implemented as PiG) constitutes the most widely used and validated biomechanical model in clinical research [44]. This study used 8 infrared tripod VICON T-20 cameras with Nexus 1.8.5 software. The system captured data at 200 Hz. Twenty-two passive retro-reflective markers (14 mm diameter) were placed on anatomical landmarks and biomechanical outcomes were calculated according to a validated modified lower body PiG model provided by the OMC software. Gait events were detected using a time-synchronized, floor-embedded force plate system (Model FP9060-15, Bertec Corporation, Ohio, USA). The OMC capture volume was calibrated prior to data collection.

Participant preparation and biomechanical model calibration
Anthropometric measurements were taken as required for the respective systems' models (height and weight for both systems; leg length from anterior superior iliac spine (ASIS) to medial malleolus and knee-and ankle width for OMC). Markers and IMUs were then placed on the participant simultaneously (Fig. 4). First, 22 OMC markers were placed on bony landmarks according to the PiG model, i.e., bilaterally on the heel (calcaneus at the same height above the plantar foot surface as the toe marker), medial and lateral malleolus, second metatarsal (mid-foot side of equinus break), shank (aligned with the ankle flexion axis), tibial tuberosity, medial and lateral knee (flexion/extension axis), lateral thigh (lower lateral one-third surface), anterior superior iliac spine and posterior superior iliac spine. Markers were not removed during any trials. IMUs were subsequently placed on the sacrum and bilaterally on the lateral thigh (lower segmental quadrant, i.e., the area of lowest muscle belly displacement during walking), shank (anterior and slightly medial to be placed along the tibia), and foot (dorsally and sufficiently proximal to the equinus break to avoid excessive IMU motion) using double-sided tape and Velcro straps. IMU foot-placements were reinforced with elastic adhesive bandage. Participants performed practice trials to familiarize themselves with testing procedures. During practice trials, starting positions for optimal force plate foot strikes were noted, and enough trials were allowed for the rater to be satisfied that a relaxed, normal gait was assumed. Static anatomical OMC calibration was subsequently performed with the participant standing on the force plate according to standard laboratory protocol (once off per participant and prior to IMU calibration). Next, the IMU model was calibrated as per the manufacturer's instructions by having the participant stand stationary in a neutral reference posture. The calibration was performed on a 30 cm-high wooden platform to mitigate potential floor-based magnetic distortions. As frequent IMU calibration within test series is recommended [45] to avoid drift over time, the IMU system was calibrated repeatedly (directly prior to each gait trial). We previously demonstrated within-rater

Gait analysis protocol
Each session consisted of a neutral-pose IMU calibration, directly followed by a barefooted walking trial during which IMU and OMC data were recorded simultaneously. Participants had to walk at a self-selected usual speed along a straight 10-m walkway with the force plate system embedded midway. Participants started walking approximately 1 m before a taped line on the floor and ended after crossing a second line. Time synchronization between the two systems was performed automatically using alignment of a hardwire synchronization pulse (5 V TTL signal). A gait trial was deemed successful if the participant's entire landing foot contacted at least one force plate without obvious targeting. Gait trials were performed in the same direction each time and after each trial, the participant returned to the wooden platform immediately for the next IMU calibration. Trials continued until good-quality data for 6 trials (3 left-and 3 right-footed force plate strikes) were obtained.

Data processing
The IMU system software automatically filtered raw data using a robust fusion algorithm (Kalman filter) optimized for IMU data. Angular orientations were estimated at IMU level by combining the elemental sensor component axes readings into four element quaternion values. Segment dimensions of the model were calculated in the IMU software using participant height to estimate anthropometric dimensions. For the determination of distance-related outcomes, the following applied: the IMU software estimated model translation over the ground using a forward kinematics technique together with sequential pinning of the foot segments onto the ground during the contact phase. The bone segment lengths on the biomechanical model were scaled to the participant dimensions. Then, using their measured orientations and known lengths, the interconnected lower limb segments were positioned in space in a kinematic chain, which translates the model forwards from foot contact to subsequent foot contact. Outcomes such as step length were then extracted from the position of virtual landmarks on the foot segments. Pre-processing of OMC gait trials was done in Nexus software. Marker trajectories were reconstructed and labeled using standard functions, then smoothed with a fourthorder, zero-lag low-pass Butterworth filter (6 Hz cut-off ) [46]. Joint and segment kinematics were calculated using the standard dynamic PiG pipeline, which determines hip joint centers using the Davis equations [47]. Knee axis estimation was performed by optimizing the thigh-rotation offset parameter during gait [48], and ankle axis estimation by determining the shank-rotation offset parameter during the static trial using medial and lateral malleolus markers. OMC gait events were detected from force plate data (20 N threshold).
Data recorded in the IMU and OMC software were, respectively, exported to single .csv and .c3d files and imported into MATLAB software (R2017a, MathWorks). In cases where a gait trial contained more than one complete and valid gait cycle for one or both legs, only the gait cycle for each leg judged to contain the best data quality was retained