Fig. 6

Immunohistochemical staining of calvarial bone defects at 8 weeks. A Immunohistochemical staining against anti-osteocalcin (OCN) and anti-osteopontin (OPN) was performed to observe osteogenesis in calvarial defects. Brown color represents positive staining. Scale bar = 200 µm (×100 images), and 50 µm (×400 images in the dotted line box). B, C OCN and OPN-positive area were calculated by ImageJ software. p < 0.05 compared to HAp (*), Qct (†), and BMP-2 (‡) groups