Fig. 3From: Restoration of Cullin3 gene expression enhances the improved effects of sonic hedgehog signaling activation for hypertension and attenuates the dysfunction of vascular smooth muscle cellsUpregulation of CUL3 expression combined with activation of SHH signaling protected against Ang II-induced VSMCs damage. A Detection of CUL3 mRNA expression with RT-qPCR. ***P < 0.001 vs. NC. B Cell viability was tested with a CCK-8 assay. C Measurement of cell migration using Transwell migration assay. Magnification, × 100. D Cell apoptosis was assessed by TUNEL staining. Magnification, × 200. E ROS level was determined with commercial available kit. ***P < 0.001 vs. control; #P < 0.05, ###P < 0.001 vs. Ang II; △P < 0.05, △△△P < 0.001 vs. Ang II + NC; $$$P < 0.001 vs. Ang II + NC + SAGBack to article page