Fig. 2From: Restoration of Cullin3 gene expression enhances the improved effects of sonic hedgehog signaling activation for hypertension and attenuates the dysfunction of vascular smooth muscle cellsInhibition of SHH signaling affected the proliferation, migration, oxidative stress and apoptosis of VSMCs induced by Ang II. A Assessment of cell viability with a CCK-8 assay. B Determination of cell migration using Transwell migration assay. Magnification, × 100. C Analysis of cell apoptosis by TUNEL staining. Magnification, × 200. D The level of ROS was examined with a commercial available kit. E Expression of proteins related to SHH signaling was evaluated with western blot assay. *P < 0.05, P < 0.001 vs. control; #P < 0.05, ###P < 0.001 vs. Ang IIBack to article page