Fig. 6

Expression of ITGBL1 in GC cells and silencing of ITGBL1 promoted apoptosis and inhibited the migration and invasion of MGC803 cells. A Results of qRT–PCR for ITGBL1 mRNA expression in GC cell lines relative to GES-1. B, C Results of qRT–PCR and Western blot for the silencing of ITGBL1. GAPDH was used as an internal control. D, E Representative images and quantification results for flow cytometry to evaluate apoptosis induction by ITGBL1 in MGC803 cells transfected with or without ITGBL1–Sh3. F Representative images for Transwell assay and quantification results of Transwell migration. Migrated cells were analyzed using ImageJ. G Representative images of Transwell invasion assay and quantitative analysis of invasive cells using Image J. The results showed that the knockdown of ITGBL1 suppressed cell migration and invasion. ^*P < 0.05, ^**P < 0.01