Fig. 6

The regulation of SIRT1 inhibitor EX527 on the apoptosis of LPS-induced A549 cells treated with wogonin. A Western blot analyzed the expression of proteins involved in SIRT1-mediated HMGB1 deacetylation in LPS-induced A549 cells treated with wogonin and EX527. B The viability LPS-induced A549 cells treated with wogonin and EX527 was detected by CCK-8 and LDH assays. C The expression of apoptosis-related proteins in LPS-induced A549 cells treated with wogonin and EX527 was tested by western blot analysis. Results were generated from three independent experiments (n = 3). ***P < 0.001 vs. control; ^##P < 0.01, ^###P < 0.001 vs. LPS; ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 vs. LPS + wogonin