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Fig. 4 | BioMedical Engineering OnLine

Fig. 4

From: Protective effects of wogonin on lipopolysaccharide-induced inflammation and apoptosis of lung epithelial cells and its possible mechanisms

Fig. 4

Effect of wogonin on the inflammation and oxidative stress of LPS-induced A549 cells. The mRNA expression levels of A TNF-α, B IL-6 and C IL-1β in LPS-induced A549 cells treated with wogonin were assessed by RT-qPCR. ELISA kits were used to evaluate the concentrations of D TNF-α, E IL-1β and F IL-6 in LPS-induced A549 cells treated with wogonin. G The protein expression levels of inflammation-related markers were examined with western blot analysis in LPS-induced A549 cells treated with wogonin. The activities of H SOD, I GSH-Px and the levels of J MDA, K ROS were evaluated by kits. Results were generated from three independent experiments (n = 3). ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. LPS + 5 μM wogonin; @@P < 0.01, @@@P < 0.001 vs. LPS + 10 μM wogonin

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