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Fig. 3 | BioMedical Engineering OnLine

Fig. 3

From: Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research

Fig. 3

Relative expression levels normalized by 13 types of single- and multiple-RG combinations. Two target genes including MAPK3 (A) and MAPK9 (B) were determined in breast cancer (BC) tissue samples (C_HR + HER −, P_HR + HER −, and benign tumor), while FAAH (C) and HIF1A (D), were determined in BC cell lines (MCF-7, MCF-10A, and MDA-MD-231). Relative expression levels of each target gene were normalized by the most stable single RGs or multiple-RG combinations (SF1 + THRAP3 + TRA2B + RHOA + QRICH1, SF1 + THRAP3 + TRA2B, THRAP3 + RHOA + QRICH1, SF1 + THRAP3, TRA2B + RHOA, SF1, THRAP3, TRA2B, RHOA, and QRICH1) and the least stable single RGs or multiple-RG combinations (ACTB, GAPDH, and ACTB + GAPDH). The error bars represent the SEM, and the independent-sample t-test was performed between any two groups, *P < 0.05, **P < 0.01, n = 6 for each BC tissue group, n = 3 for each BC cell strain. The patterns of target gene expression were different between those normalized by the most stable RGs and those normalized by the least stable RGs

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