Fig. 5

Topotecan treatment blocked the inhibitory effects of HIF-1α overexpression on the apoptosis in LPS-induced H9c2 cells. A Cell viability was detected using a CCK-8 assay. B, C Cell apoptosis ratio was evaluated with flow cytometry. D Western blotting was employed to examine the expression of apoptosis-related proteins. ^**P < 0.01, ^***P < 0.001 vs. control; ^##P < 0.01, ^###P < 0.001 vs. LPS; ^△P < 0.05, ^△△△P < 0.001 vs. LPS + pcDNA-HIF-1α