Fig. 4From: Hypoxia-inducible transcription factor-1α inhibition by topotecan protects against lipopolysaccharide-induced inflammation and apoptosis of cardiomyocytesTopotecan intervention abrogated the impact of HIF-1α-upregulation on the inflammation in LPS-induced H9c2 cells. A Cell viability was measured using a CCK-8 assay after topotecan treatment. B The expression of HIF-1α was tested by means of western blot analysis after topotecan intervention. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control. C–E ELISA kits were used to determine the levels of TNF-α, IL-6 and IL-8. ***P < 0.001 vs. control; ###P < 0.001 vs. LPS; △△△P < 0.001 vs. LPS + pcDNA-HIF-1αBack to article page