Fig. 2From: Hypoxia-inducible transcription factor-1α inhibition by topotecan protects against lipopolysaccharide-induced inflammation and apoptosis of cardiomyocytesHIF-1α expression was markedly upregulated in LPS-stimulated H9c2 cells, and HIF-1α ablation ameliorated LPS-induced inflammatory responses of cardiomyocytes H9c2 cells. A The expression of HIF-1α was determined with RT-qPCR after LPS exposure. ***P < 0.001 vs. control. B RT-qPCR was used to evaluate the level of HIF-1α following transfection. ***P < 0.001 vs. siRNA-NC; ###P < 0.001 vs. pcDNA-NC. C–E The secretion levels of TNF‐α, IL‐6 and IL‐8 were tested by means of ELISA kits. ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPSBack to article page