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Table 1 Pulsing protocols for gene electrotransfer in 3D model

From: The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model

Protocol Electric pulse parameters
HV 1 5 × 1 ms; 0.8 kV/cm; 1 Hz
HV 2 8 × 200 µs; 0.8 kV/cm; 1 Hz
LV 1 1 × 100 ms; 75 V/cm
LV 2 1 × 100 ms; 150 V/cm
Single polarity pulses—SP 8 × 1 ms; 0.8 kV/cm; 1 Hz
Orthogonal both polarities—OBP 8 × 1 ms; 0.8 kV/cm; 1 Hz
  1. The time lag between HV and LV pulse was always 20 ms