Fig. 4

GATA3 activated NCAPH expression in OC cells by binding to the NCAPH promoter. A GATA3 binding with the promoter of NCAPH was predicated by JASPAR databases. B NCAPH level in OC tissues was tested by TNMplot (https://www.tnmplot.com/). C, D NCAPH expression in several OC cell lines (SKOV3, OVCAR3, A2780, and CAOV3) and one human ovarian epithelial cell line HOSEpiC was evaluated with RT-qPCR and Western blot analysis. ^***P < 0.001 vs. HOSEpiC. (E, F) GATA3 expression was measured using RT-qPCR and Western blotting after GATA3 overexpression. Data were obtained from three independent experiments. ^**P < 0.01, ^***P < 0.001 vs. Ov-NC. G, H The binding between GATA6 and the promoter regions (S1 and S2) was determined by luciferase reporter assays. Results were generated from three independent experiments. ^***P < 0.001 vs. Ov-NC. I Chromosomal immunoprecipitation assay was used to detect the direct binding of GATA3 to NCAPH promoter. All experiments were performed in triplicates. ^***P < 0.001 vs. IgG. J, K NCAPH levels were assessed by RT-qPCR and Western blot analysis. ^***P < 0.001 vs. si-NC; ^###P < 0.001 vs. si-NCAPH#2+Ov-NC