Thymoquinone loading into hydroxyapatite/alginate scaffolds accelerated the osteogenic differentiation of the mesenchymal stem cells

Table 1 The promoter sequences used

Name	Primer sequence (5′ → 3′)	Melting point °C
Collagen 1	Forward: CGGCTCCTGCTCCTCTTAG	65
Collagen 1	Reverse: GGGCTCGGGTTTCCACACG	65
Osteocalcin	Forward: CCTCACACTCCTCGCCCTA	63
Osteocalcin	Reverse: TCTTCACTACCTCGCTGCC	63
Osteopontin	Forward: CTCAGCCAAACGCCGACCAA	65
Osteopontin	Reverse: TCCTCAGAACTTCCAGAATCAGCCT	65
β-Actin	Forward: GCCTTTGCCGATCCGC	55
β-Actin	Reverse: GCCGTAGCCGTTGTCG	55
GAPDH	Forward: GCAAGAGCACAAGAGGAAGA	57
GAPDH	Reverse: ACTGTGAGGAGGGGAGATTC	57

ISSN: 1475-925X