Fig. 8
Expression of A collagen I, B osteopontin, C osteocalcin genes in mesenchymal cells cultured on hydroxyapatite-alginate scaffolds in the presence and absence of osteogenic medium and thymoquinone. *Significant difference between the groups (*P < 0.05, **P < 0.01, ***P < 0.001). (P = 0.001 for 3D + TQ + OM versus 3D + TQ − OM, P = 0.0008 for 3D − TQ + OM versus 3D − TQ − OM on 7 day; P < 0.0001 for all groups with/without OM on day 28 in collagen 1 expression), (P = 0.003 for 3D + TQ + OM versus 3D + TQ − OM, P = 0.03 for 3D − TQ + OM versus 3D − TQ − OM on 7 day; P < 0.0001 for 3D + TQ + OM versus 3D + TQ − OM, P = 0.0005 for 3D − TQ + OM versus 3D − TQ − OM on day 28 in osteopontin expression), and (P = 0.005 for 3D + TQ + OM versus 3D + TQ − OM, P = 0.01 for 3D − TQ + OM versus 3D − TQ − OM on 7 day; P < 0.0001 for all groups with/ without OM on day 28 in osteocalcin expression)