Fig. 5

Biocompatibility of the Co (0, 1, 2.5, 5, 10) scaffolds. A Cytotoxicity analysis of the Co (0, 1, 2.5, 5, 10) scaffolds; less than 70% cell viability was considered cytotoxic. B The proliferation of rBMSCs cultured on the Co (0, 1, 2.5, 5) scaffolds. The data are expressed as the mean ± SD. *p < 0.05 and **p < 0.01, comparison with the Co0 group; ^#p < 0.05, comparison among the Co (1, 2.5, 5) groups. C SEM micrographs of rBMSCs morphology cultured on Co (0, 1, 2.5, 5) scaffolds for 3 days (scale bars = 10 µm). D CLSM immunofluorescence micrographs of rBMSCs morphology cultured on the Co (0, 1, 2.5, 5) scaffolds for 3 days and 6 days. Cell skeleton staining with phalloidin (green). Nuclear staining with DAPI (blue) (scale bars = 100 µm)