Fig. 4From: Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expressionCPSF6 as an RNA binding protein can bind and regulate BTG2 expression. A BTG2 mRNA expression in Huh-7 cells was detected by RT-qPCR analysis. B BTG2 protein expression in Huh-7 cells was determined by Western blot analysis. *P < 0.05 and ***P < 0.001 vs. THLE-3 group. C RIP assay confirmed that CPSF6 was interacted with BTG2. **P < 0.01 vs. Input group. ##P < 0.01 vs. IgG group. D BTG2 mRNA expression in Huh-7 cells transfected with shRNA-BTG2-1/2 was detected by RT-qPCR analysis. E BTG2 protein expression in Huh-7 cells transfected with shRNA-BTG2-1/2 was determined by Western blot analysis. ***P < 0.001 vs. Control group. ###P < 0.001 vs. shRNA-NC group. F BTG2 mRNA expression in Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was detected by RT-qPCR analysis. G BTG2 mRNA expression in Huh-7 cells transfected with sh-CPSF6-2 and shRNA-BTG2-1 was detected by Western blot analysis. *P < 0.05 and ***P < 0.001 vs. Control group. ###P < 0.001 vs. sh-NC group. $$P < 0.01 and $$$P < 0.001 vs. sh-CPSF6-2 group. N = 3Back to article page