Fig. 5

Real-world comparison of pressure measured from VPS versus separate pressure meter. Data are presented from three different female mice (mice A, B, C). To compare the inter-rater reliability between the VPS pressure transducer and a separate pressure transducer in our analog-to-digital converter, data from multiple trials a at 30 mmHg or b 60 mmHg are plotted against each other for the two transducers. ICC (95% confidence intervals) and P values are provided for each graph. There is excellent reliability between the two transducers. Variability between trials within mice is due to variability in the manual adjustment of pressure that was done as trials alternated between 30 and 60 mmHg. Pressure target during manual adjustment was completed using a separate analog sphygmometer with a ± 3 mmHg error. Large open symbols show mean pressure for that particular animal across all 10 trials