Fig. 1
From: Computational flow cytometric analysis to detect epidermal subpopulations in human skin
A representative sample shows the subgroup phenotype by manual analysis. a The manual gating strategy for the panel. This strategy removes debris, doublets, low viability cells, and CD45-positive cells. The cellular phenotype was displayed in a serial biaxial gates. b, c The phenotypes of PI to PV. d The heatmap of each subset percentage of live epidermal cells. The first four lines indicated the immunophenotype shown in B. Left row indicated the body sites as ear, thorax and abdomen. Logarithmic conversion of percentage values was performed. e Volcano plot of subsets which have statistical difference between body sites