|
Microarray platforms
|
Direct hybridization with probes present in the platform
|
Potential scalability; quantitative results; few steps until final results
|
Needed of specialized professionals and equipment; requires the previous setup of miRNA panel; elevated costs of customized panel
|
fmol
|
[178, 179]
|
|
qPCR
|
miRNA amplification and quantification; results were detected using optical sensors
|
Widespread technique; quantitative results; few steps until final results
|
Needed of specialized professionals and equipment; time until final results; requires the previous setup of miRNA panel
|
fmol
|
[162]
|
|
Next-generation sequencing
|
Direct sequencing of miRNAs present in the sample
|
Quantification of all miRNAs in the sample; high accuracy
|
Elevated operational cost of analysis; needed of specialized professionals and equipment; time until final results
|
fmol
|
[180, 181]
|
|
Northern blot
|
Hybridization with probes and detected according to their sequences and molecular weight
|
Widespread technique
|
Semi-quantitative method; laborious and multiple steps protocol
|
pmol
|
[182, 183]
|
|
Isothermal amplification
|
miRNA amplification and quantification; results were detected using optical sensor
|
Low-cost technique; potential scalability
|
Nonspecific background amplification; multiple steps protocol; Scalability using clinical samples
|
fmol
|
[184,185,186]
|
