Fig. 6

Decellularized thyroid gland scaffold biological function evaluation. Quantitative assay of cytokines in the DTG scaffolds (a). Effect of DTG scaffolds on cell proliferative activity (b). Recellularization of the DTG scaffolds (c, d). The DTG scaffolds were seeded with HTFCs to generate a co-culture system at 3 days (c) and 7 days (d). Immunostaining showed the TPO weak expression at 3 days and significantly reduced at 7 day. Data are shown as mean ± SD.*p < 0.05. Scale bar = 50 µm