Fig. 5

Nrf2 is a functional target of miR-153. a The putative binding of miR-153 with the wild-type or mutated 3′-UTR region of Nrf2 mRNA is shown. b HEK 293T cells were transfected with rat miR-153 mimics, miR-NC, pRT-TK Renilla, and Luc-Nrf2-wt or Luc-Nrf2-mut. After 48 h of incubation, relative luciferase activities were measured. Three independent experiments have been carried out. Sprague–Dawley rats were received the intramyocardial injection of saline, adenovirus-expressing siRNA-nonsense control (siRNA-NC), a combination of siRNA-NC and anti-miR-153 (anti-miR-153 + siRNA-NC), or a combination of siRNA-Nrf2 and anti-miR-153 (anti-miR-153 + siRNA-Nrf2). c The mRNA levels of Nrf2 in four groups of rats were measured by qRT-PCR. d, e The protein levels of Nrf2 in four groups of rats were measured by western blotting assay (d). The results were quantified by image J software (e). The mRNA levels of caspase-3 (f), Bax (g), and Bcl-2 (h) in four groups of rats were measured by qRT-PCR. n = 8 for each group. Experiments have repeated for three times independently. Data are presented as mean ± SD. **p < 0.01 and ***p < 0.001 compared to sham group. ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001