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Fig. 1 | BioMedical Engineering OnLine

Fig. 1

From: Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

Fig. 1

Simplified schematic representation of the E. coli strain DH5α transfection. a Recombinant plasmid (pGL3-luc/pbr biosensor). b Recombinant plasmid (pGL3-luc/cad biosensor). pGL3-luc/pbr biosensor and pGL3-luc/Cad biosensor were transferred to the E. coli strain DH5α using the chemical method of CaCl2 and then were screened using selective plates containing antibiotic ampicillin. c Sequencing and integrity of synthesis sequence. d PGL3-luc/pbr biosensor pGL3-luc/cad biosensor. The promoter region was sequenced in the received plasmid

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BioMedical Engineering OnLine

ISSN: 1475-925X

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